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A novel LRR-only protein mediates bacterial proliferation in hemolymph through regulating expression of antimicrobial peptides in mollusk Chlamys farreri
Wang, Mengqiang1,3; Wang, Baojie1; Liu, Mei1; Jiang, Keyong1; Wang, Lei1,2,4
2019-03-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume92Pages:223-229
Corresponding AuthorWang, Lei(wanglei@qdio.ac.cn)
AbstractLeucine-rich repeat (LRR)-only proteins are involved in innate immune responses through mediating protein-ligand or protein-protein interactions, yet the exact roles of most LRR-only proteins in invertebrates are not well documented. In the present study, a novel LRR-only protein (designated CfLRRop-7) was identified in Zhikong scallop Chlamys farreri. The full-length cDNA sequence of CfLRRop-7 was 1463 bp and contained an open reading frame of 1086 bp, which encoded a protein of 361 amino acids. Five LRR motifs with a conserved signature sequence LxxLxLxxNxL were identified in the predicted protein sequence. The expression of CfLRRop-7 was particularly high in hemocytes. The expression of CfLRRop-7 was relatively high in oocytes and embryos during the ontogenesis of scallops. CfLRRop-7 expression changed in hemocytes in response to stimulation with different microbes, including Vibrio splendidus, Staphylococcus aureus and Pichia pastoris. CfLRRop-7 recognized five kinds of ligands/agonists. CfLRRop-7 recombinant protein inhibited bacterial proliferation in hemolymph and induced lysozyme activity in serum. After knocking down CfLRRop-7, the mRNA expression of selected antimicrobial peptides was reduced. All these results indicated that CfLRRop-7 might be a potential pattern recognition receptor that recognizes various pathogen associated molecular patterns, and regulates antibacterial immunity in scallops.
KeywordInnate immunity LRR-only protein Hemolymph microbiome
DOI10.1016/j.dci.2018.11.013
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41306156] ; National Natural Science Foundation of China[U1706209] ; Aoshan Innovation Project in Science and Technology from National Laboratory for Marine Science and Technology[2016ASKJ07] ; Science and Technology Service Network Plan (STS) Major Deployment Project[KFZD-SW-106] ; STS Regional Centre Project (Fujian province) from Chinese Academy of Sciences
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000458596200023
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155602
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Lei
Affiliation1.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
2.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Peoples R China
4.Chinese Acad Sci, CAS Ctr Ocean Mega Sci, Qingdao 266400, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Mengqiang,Wang, Baojie,Liu, Mei,et al. A novel LRR-only protein mediates bacterial proliferation in hemolymph through regulating expression of antimicrobial peptides in mollusk Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,92:223-229.
APA Wang, Mengqiang,Wang, Baojie,Liu, Mei,Jiang, Keyong,&Wang, Lei.(2019).A novel LRR-only protein mediates bacterial proliferation in hemolymph through regulating expression of antimicrobial peptides in mollusk Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,92,223-229.
MLA Wang, Mengqiang,et al."A novel LRR-only protein mediates bacterial proliferation in hemolymph through regulating expression of antimicrobial peptides in mollusk Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 92(2019):223-229.
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