Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| A novel LRR-only protein mediates bacterial proliferation in hemolymph through regulating expression of antimicrobial peptides in mollusk Chlamys farreri | |
Wang, Mengqiang1,3 ; Wang, Baojie1; Liu, Mei1; Jiang, Keyong1; Wang, Lei1,2,4
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| 2019-03-01 | |
| Source Publication | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| ISSN | 0145-305X |
| Volume | 92Pages:223-229 |
| Corresponding Author | Wang, Lei(wanglei@qdio.ac.cn) |
| Abstract | Leucine-rich repeat (LRR)-only proteins are involved in innate immune responses through mediating protein-ligand or protein-protein interactions, yet the exact roles of most LRR-only proteins in invertebrates are not well documented. In the present study, a novel LRR-only protein (designated CfLRRop-7) was identified in Zhikong scallop Chlamys farreri. The full-length cDNA sequence of CfLRRop-7 was 1463 bp and contained an open reading frame of 1086 bp, which encoded a protein of 361 amino acids. Five LRR motifs with a conserved signature sequence LxxLxLxxNxL were identified in the predicted protein sequence. The expression of CfLRRop-7 was particularly high in hemocytes. The expression of CfLRRop-7 was relatively high in oocytes and embryos during the ontogenesis of scallops. CfLRRop-7 expression changed in hemocytes in response to stimulation with different microbes, including Vibrio splendidus, Staphylococcus aureus and Pichia pastoris. CfLRRop-7 recognized five kinds of ligands/agonists. CfLRRop-7 recombinant protein inhibited bacterial proliferation in hemolymph and induced lysozyme activity in serum. After knocking down CfLRRop-7, the mRNA expression of selected antimicrobial peptides was reduced. All these results indicated that CfLRRop-7 might be a potential pattern recognition receptor that recognizes various pathogen associated molecular patterns, and regulates antibacterial immunity in scallops. |
| Keyword | Innate immunity LRR-only protein Hemolymph microbiome |
| DOI | 10.1016/j.dci.2018.11.013 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | STS Regional Centre Project (Fujian province) from Chinese Academy of Sciences ; Science and Technology Service Network Plan (STS) Major Deployment Project[KFZD-SW-106] ; Aoshan Innovation Project in Science and Technology from National Laboratory for Marine Science and Technology[2016ASKJ07] ; National Natural Science Foundation of China[U1706209] ; National Natural Science Foundation of China[41306156] ; National Natural Science Foundation of China[41306156] ; National Natural Science Foundation of China[U1706209] ; Aoshan Innovation Project in Science and Technology from National Laboratory for Marine Science and Technology[2016ASKJ07] ; Science and Technology Service Network Plan (STS) Major Deployment Project[KFZD-SW-106] ; STS Regional Centre Project (Fujian province) from Chinese Academy of Sciences |
| WOS Research Area | Fisheries ; Immunology ; Zoology |
| WOS Subject | Fisheries ; Immunology ; Zoology |
| WOS ID | WOS:000458596200023 |
| Publisher | ELSEVIER SCI LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/155601 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Wang, Lei |
| Affiliation | 1.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, CAS Ctr Ocean Mega Sci, Qingdao 266400, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Wang, Mengqiang,Wang, Baojie,Liu, Mei,et al. A novel LRR-only protein mediates bacterial proliferation in hemolymph through regulating expression of antimicrobial peptides in mollusk Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,92:223-229. |
| APA | Wang, Mengqiang,Wang, Baojie,Liu, Mei,Jiang, Keyong,&Wang, Lei.(2019).A novel LRR-only protein mediates bacterial proliferation in hemolymph through regulating expression of antimicrobial peptides in mollusk Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,92,223-229. |
| MLA | Wang, Mengqiang,et al."A novel LRR-only protein mediates bacterial proliferation in hemolymph through regulating expression of antimicrobial peptides in mollusk Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 92(2019):223-229. |
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| A novel LRR-only pro（1310KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | View | |
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