IOCAS-IR  > 实验海洋生物学重点实验室
Gill symbionts of the cold-seep mussel Bathymodiolus platifrons: Composition, environmental dependency and immune control
Yu, Jiajia1,3; Wang, Minxiao1,2; Liu, Baozhong1,2; Yue, Xin1; Li, Chaolun1,2
2019-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume86Pages:246-252
Corresponding AuthorYue, Xin(yuexin@qdio.ac.cn) ; Li, Chaolun(lcl@qdio.ac.cn)
AbstractDeep-sea Bathymodiolus mussels depend on the organic carbon supplied by symbionts inside their gills. In this study, optimized methods of quantitative real-time PCR and fluorescence in situ hybridization targeted to both mRNA and 16S rRNA were used to investigate the gill symbionts of the cold-seep mussel Bathymodiolus platifrons, including species composition, environmental dependency and immune control by the host. Our results showed that methanotrophs were the major symbiotic bacteria in the gills of B. platifrons, while thiotrophs were scarce. In the mussels freshly collected from the deep sea, methanotrophs were housed in bacteriocytes in a unique circular pattern, and a lysosome-related gene (VAMP) encoding a vesicle-associated membrane protein was expressed at a high level and presented exactly where the methanotrophs occurred. After the mussels were reared for three months in aquaria without methane supply, the abundance of methanotrophs decreased significantly and their circle-shaped distribution pattern disappeared; in addition, the expression of VAMP decreased significantly. These results suggest that the symbiosis between B. platifrons and methanotrophs is influenced by the environment and that the lysosomal system plays an important immune role in controlling the abundance of endosymbionts in host. This study provides a reliable method for investigating symbionts in deep-sea mussels and enriches the knowledge about symbionts in B. platifrons.
KeywordBathymodiolus platifrons Methanotrophs FISH qRT-PCR Lysosomal system
DOI10.1016/j.fsi.2018.11.041
Indexed BySCI
Language英语
Funding ProjectStrategic Priority Research Program of the Chinese Academy of Sciences[XDA11030202] ; International Partnership for Innovative Team Program from the Chinese Academy of Sciences[20140491526] ; Youth Foundation Project[YQ2018NO07] ; Scientific and Technological Innovation Project from Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000459528400029
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155576
Collection实验海洋生物学重点实验室
Corresponding AuthorYue, Xin; Li, Chaolun
Affiliation1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266000, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yu, Jiajia,Wang, Minxiao,Liu, Baozhong,et al. Gill symbionts of the cold-seep mussel Bathymodiolus platifrons: Composition, environmental dependency and immune control[J]. FISH & SHELLFISH IMMUNOLOGY,2019,86:246-252.
APA Yu, Jiajia,Wang, Minxiao,Liu, Baozhong,Yue, Xin,&Li, Chaolun.(2019).Gill symbionts of the cold-seep mussel Bathymodiolus platifrons: Composition, environmental dependency and immune control.FISH & SHELLFISH IMMUNOLOGY,86,246-252.
MLA Yu, Jiajia,et al."Gill symbionts of the cold-seep mussel Bathymodiolus platifrons: Composition, environmental dependency and immune control".FISH & SHELLFISH IMMUNOLOGY 86(2019):246-252.
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