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Comparative characterization of putative chitin deacetylases from Phaeodactylum tricornutum and Thalassiosira pseudonana highlights the potential for distinct chitin-based metabolic processes in diatoms
Shao, Zhanru1,2,3,4; Thomas, Yann2; Hembach, Lea5; Xing, Xiaohui6,7,8; Duan, Delin1,3,4; Moerschbacher, Bruno M.5; Bulone, Vincent6,7,8; Tirichine, Leila2; Bowler, Chris2
2019-03-01
Source PublicationNEW PHYTOLOGIST
ISSN0028-646X
Volume221Issue:4Pages:1890-1905
Corresponding AuthorDuan, Delin(dlduan@qdio.ac.cn) ; Bowler, Chris(cbowler@biologie.ens.fr)
AbstractChitin is generally considered to be present in centric diatoms but not in pennate species. Many aspects of chitin biosynthetic pathways have not been explored in diatoms. We retrieved chitin metabolic genes from pennate (Phaeodactylum tricornutum) and centric (Thalassiosira pseudonana) diatom genomes. Chitin deacetylase (CDA) genes from each genome (PtCDA and TpCDA) were overexpressed in P. tricornutum. We performed comparative analysis of their sequence structure, phylogeny, transcriptional profiles, localization and enzymatic activities. The chitin relevant proteins show complex subcellular compartmentation. PtCDA was likely acquired by horizontal gene transfer from prokaryotes, whereas TpCDA has closer relationships with sequences in Opisthokonta. Using transgenic P. tricornutum lines expressing CDA-green fluorescent protein (GFP) fusion proteins, PtCDA predominantly localizes to Golgi apparatus whereas TpCDA localizes to endoplasmic reticulum/chloroplast endoplasmic reticulum membrane. CDA-GFP overexpression upregulated the transcription of chitin synthases and potentially enhanced the ability of chitin synthesis. Although both CDAs are active on GlcNAc(5), TpCDA is more active on the highly acetylated chitin polymer DA60. We have addressed the ambiguous characters of CDAs from P. tricornutum and T. pseudonana. Differences in localization, evolution, expression and activities provide explanations underlying the greater potential of centric diatoms for chitin biosynthesis. This study paves the way for in vitro applications of novel CDAs.
Keywordchitin chitin deacetylase chitosan enzymatic activity gene transformation Phaeodactylum tricornutum subcellular localization Thalassiosira pseudonana
DOI10.1111/nph.15510
Indexed BySCI
Language英语
Funding ProjectEuropean Union's Seventh Framework Programme[613931] ; European Research Council Advanced Award 'Diatomite' ; French Government 'Investissements d'Avenir' programme MEMO LIFE[ANR-10-LABX-54] ; French Government 'Investissements d'Avenir' programme Paris Sciences et Lettres[ANR-1253 11-IDEX-0001-02] ; National Natural Science Foundation of China[41806175]
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000458259600023
PublisherWILEY
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155573
Collection实验海洋生物学重点实验室
Corresponding AuthorDuan, Delin; Bowler, Chris
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.PSL Res Univ, Ecole Normale Super, IBENS, CNRS,INSERM, F-75005 Paris, France
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
5.Westphalian Wilhelms Univ Munster, Inst Plant Biol & Biotechnol, D-48143 Munster, Germany
6.Univ Adelaide, Sch Agr Food & Wine, Australian Res Council Ctr Excellence Plant Cell, Waite Campus, Urrbrae, SA 5064, Australia
7.Univ Adelaide, Sch Agr Food & Wine, Adelaide Glyc, Waite Campus, Urrbrae, SA 5064, Australia
8.Royal Inst Technol KTH, AlbaNova Univ Ctr, Sch Biotechnol, Div Glycosci, SE-10691 Stockholm, Sweden
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Shao, Zhanru,Thomas, Yann,Hembach, Lea,et al. Comparative characterization of putative chitin deacetylases from Phaeodactylum tricornutum and Thalassiosira pseudonana highlights the potential for distinct chitin-based metabolic processes in diatoms[J]. NEW PHYTOLOGIST,2019,221(4):1890-1905.
APA Shao, Zhanru.,Thomas, Yann.,Hembach, Lea.,Xing, Xiaohui.,Duan, Delin.,...&Bowler, Chris.(2019).Comparative characterization of putative chitin deacetylases from Phaeodactylum tricornutum and Thalassiosira pseudonana highlights the potential for distinct chitin-based metabolic processes in diatoms.NEW PHYTOLOGIST,221(4),1890-1905.
MLA Shao, Zhanru,et al."Comparative characterization of putative chitin deacetylases from Phaeodactylum tricornutum and Thalassiosira pseudonana highlights the potential for distinct chitin-based metabolic processes in diatoms".NEW PHYTOLOGIST 221.4(2019):1890-1905.
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