Comparative characterization of putative chitin deacetylases from Phaeodactylum tricornutum and Thalassiosira pseudonana highlights the potential for distinct chitin-based metabolic processes in diatoms

doi:10.1111/nph.15510

IOCAS-IR > 实验海洋生物学重点实验室

	Comparative characterization of putative chitin deacetylases from Phaeodactylum tricornutum and Thalassiosira pseudonana highlights the potential for distinct chitin-based metabolic processes in diatoms
	Shao, Zhanru 1,2,3,4; Thomas, Yann 2; Hembach, Lea 5; Xing, Xiaohui 6,7,8; Duan, Delin 1,3,4; Moerschbacher, Bruno M.5; Bulone, Vincent 6,7,8; Tirichine, Leila 2; Bowler, Chris 2
	2019-03-01
发表期刊	NEW PHYTOLOGIST
ISSN	0028-646X
卷号	221 期号:4 页码:1890-1905
通讯作者	Duan, Delin(dlduan@qdio.ac.cn) ; Bowler, Chris(cbowler@biologie.ens.fr)
摘要	Chitin is generally considered to be present in centric diatoms but not in pennate species. Many aspects of chitin biosynthetic pathways have not been explored in diatoms. We retrieved chitin metabolic genes from pennate (Phaeodactylum tricornutum) and centric (Thalassiosira pseudonana) diatom genomes. Chitin deacetylase (CDA) genes from each genome (PtCDA and TpCDA) were overexpressed in P. tricornutum. We performed comparative analysis of their sequence structure, phylogeny, transcriptional profiles, localization and enzymatic activities. The chitin relevant proteins show complex subcellular compartmentation. PtCDA was likely acquired by horizontal gene transfer from prokaryotes, whereas TpCDA has closer relationships with sequences in Opisthokonta. Using transgenic P. tricornutum lines expressing CDA-green fluorescent protein (GFP) fusion proteins, PtCDA predominantly localizes to Golgi apparatus whereas TpCDA localizes to endoplasmic reticulum/chloroplast endoplasmic reticulum membrane. CDA-GFP overexpression upregulated the transcription of chitin synthases and potentially enhanced the ability of chitin synthesis. Although both CDAs are active on GlcNAc(5), TpCDA is more active on the highly acetylated chitin polymer DA60. We have addressed the ambiguous characters of CDAs from P. tricornutum and T. pseudonana. Differences in localization, evolution, expression and activities provide explanations underlying the greater potential of centric diatoms for chitin biosynthesis. This study paves the way for in vitro applications of novel CDAs.
关键词	chitin chitin deacetylase chitosan enzymatic activity gene transformation Phaeodactylum tricornutum subcellular localization Thalassiosira pseudonana
DOI	10.1111/nph.15510
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41806175] ; French Government 'Investissements d'Avenir' programme Paris Sciences et Lettres[ANR-1253 11-IDEX-0001-02] ; French Government 'Investissements d'Avenir' programme MEMO LIFE[ANR-10-LABX-54] ; European Research Council Advanced Award 'Diatomite' ; European Union's Seventh Framework Programme[613931] ; European Union's Seventh Framework Programme[613931] ; European Research Council Advanced Award 'Diatomite' ; French Government 'Investissements d'Avenir' programme MEMO LIFE[ANR-10-LABX-54] ; French Government 'Investissements d'Avenir' programme Paris Sciences et Lettres[ANR-1253 11-IDEX-0001-02] ; National Natural Science Foundation of China[41806175]
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000458259600023
出版者	WILEY
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/155572
专题	实验海洋生物学重点实验室
通讯作者	Duan, Delin; Bowler, Chris
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.PSL Res Univ, Ecole Normale Super, IBENS, CNRS,INSERM, F-75005 Paris, France 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Westphalian Wilhelms Univ Munster, Inst Plant Biol & Biotechnol, D-48143 Munster, Germany 6.Univ Adelaide, Sch Agr Food & Wine, Australian Res Council Ctr Excellence Plant Cell, Waite Campus, Urrbrae, SA 5064, Australia 7.Univ Adelaide, Sch Agr Food & Wine, Adelaide Glyc, Waite Campus, Urrbrae, SA 5064, Australia 8.Royal Inst Technol KTH, AlbaNova Univ Ctr, Sch Biotechnol, Div Glycosci, SE-10691 Stockholm, Sweden
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Shao, Zhanru,Thomas, Yann,Hembach, Lea,et al. Comparative characterization of putative chitin deacetylases from Phaeodactylum tricornutum and Thalassiosira pseudonana highlights the potential for distinct chitin-based metabolic processes in diatoms[J]. NEW PHYTOLOGIST,2019,221(4):1890-1905.
APA	Shao, Zhanru.,Thomas, Yann.,Hembach, Lea.,Xing, Xiaohui.,Duan, Delin.,...&Bowler, Chris.(2019).Comparative characterization of putative chitin deacetylases from Phaeodactylum tricornutum and Thalassiosira pseudonana highlights the potential for distinct chitin-based metabolic processes in diatoms.NEW PHYTOLOGIST,221(4),1890-1905.
MLA	Shao, Zhanru,et al."Comparative characterization of putative chitin deacetylases from Phaeodactylum tricornutum and Thalassiosira pseudonana highlights the potential for distinct chitin-based metabolic processes in diatoms".NEW PHYTOLOGIST 221.4(2019):1890-1905.

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