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Wnt Signaling Pathway Linked to Intestinal Regeneration via Evolutionary Patterns and Gene Expression in the Sea Cucumber Apostichopus japonicus
Yuan, Jianbo1,2,3,4; Gao, Yi1,2,3,4; Sun, Lina1,2,3,4; Jin, Songjun1,2,3,4; Zhang, Xiaojun1,2,3,4; Liu, Chengzhang1,2,3,4; Li, Fuhua1,2,3,4; Xiang, Jianhai1,2,3,4
2019-02-19
Source PublicationFRONTIERS IN GENETICS
ISSN1664-8021
Volume10Pages:13
Corresponding AuthorYuan, Jianbo(yuanjb@qdio.ac.cn) ; Zhang, Xiaojun(xjzhang@qdio.ac.cn) ; Xiang, Jianhai(jhxiang@qdio.ac.cn)
AbstractMany echinoderms are regenerative species that exhibit exceptional regenerative capacity, and sea cucumber is a representative organism that could regenerate the whole intestine after evisceration. There are many signaling pathways participate in the regeneration process, but it is not clear which is essential for the intestinal regeneration. In this study, we performed genome-wide comprehensive analyses on these regeneration-related signaling pathways, and found the Wnt signaling pathway was one of the most conservative pathways among regenerative species. Additionally, among these signaling pathways, we found that the Wnt signaling pathway was the only one under positive selection in regenerative echinoderms, and the only one enriched by differentially expressed genes during the intestinal regeneration. Thus, it suggests both coding sequence and gene expression of the Wnt signaling pathway have been shaped by natural selection to provide the genetic architecture for intestinal regeneration. Wnt7, Fz7, and Dvl are the three positively selected genes and also happen to be three upstream genes in the Wnt signaling pathway. They are all significantly upregulated at the early stages of regeneration, which may contribute significantly to the early activation of Wnt signaling and the initiation of intestinal regeneration. Expression knockdown of Wnt7 and Dvl by RNA interference significantly inhibit intestinal extension, implying that they are essential for intestinal regeneration. As an important regeneration-related gene, the downstream gene c-Myc is also conserved and highly expressed during the whole regeneration stages, which may make the Wnt/c-Myc signaling to be an important way to promote intestinal regeneration. Therefore, it is reasonable to conclude that the Wnt signaling pathway is the chosen one to play an important role in intestinal regeneration of sea cucumbers, or even in the regeneration of other echinoderms.
Keywordsea cucumber intestinal regeneration Wnt signaling pathway positive selection natural selection
DOI10.3389/fgene.2019.00112
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41876167] ; National Natural Science Foundation of China[41776162] ; National Natural Science Foundation of China[41506189] ; China Agriculture Research system-47 (CARS-47) ; Senior User Project of RV KEXUE[KEXUE2018G19]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000459167300001
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155560
Collection实验海洋生物学重点实验室
Corresponding AuthorYuan, Jianbo; Zhang, Xiaojun; Xiang, Jianhai
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol & Marine Ecol & Envi, Qingdao, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yuan, Jianbo,Gao, Yi,Sun, Lina,et al. Wnt Signaling Pathway Linked to Intestinal Regeneration via Evolutionary Patterns and Gene Expression in the Sea Cucumber Apostichopus japonicus[J]. FRONTIERS IN GENETICS,2019,10:13.
APA Yuan, Jianbo.,Gao, Yi.,Sun, Lina.,Jin, Songjun.,Zhang, Xiaojun.,...&Xiang, Jianhai.(2019).Wnt Signaling Pathway Linked to Intestinal Regeneration via Evolutionary Patterns and Gene Expression in the Sea Cucumber Apostichopus japonicus.FRONTIERS IN GENETICS,10,13.
MLA Yuan, Jianbo,et al."Wnt Signaling Pathway Linked to Intestinal Regeneration via Evolutionary Patterns and Gene Expression in the Sea Cucumber Apostichopus japonicus".FRONTIERS IN GENETICS 10(2019):13.
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