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Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida
Liu, Peng-fei1,2,3; Du, Yishuai1,2,3; Meng, Lingjie1,2,3; Li, Xian1,2,3; Yang, Dung5; Liu, Ying4
2019-02-14
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume9Pages:12
Corresponding AuthorYang, Dung(yangdongbprc@163.com) ; Liu, Ying(yingliu@dlou.edu.cn)
AbstractAeromonas salmonicida (A. salmonicida) is a pathogenic bacterium that causes furunculosis and poses a significant global risk, particularly in economic activities such as Atlantic salmon (Salmo salar) farming. In a previous study, we identified proteins that are significantly upregulated in kidneys of Atlantic salmon challenged with A. salmonicida. Phosphoproteomic analyses were conducted to further clarify the dynamic changes in protein phosphorylation patterns triggered by bacterial infection. To our knowledge, this is the first study to characterize phosphorylation events in proteins from A. salmonicida-infected Atlantic salmon. Overall, we identified over 5635 phosphorylation sites in 3112 proteins, and 1502 up-regulated and 77 down-regulated proteins quantified as a 1.5-fold or greater change relative to control levels. Based on the combined data from proteomic and motif analyses, we hypothesize that five prospective novel kinases (VRK3, GAK, HCK, PKCd and RSK6) with common functions in inflammatory processes and cellular pathways to regulate apoptosis and the cytoskeleton could serve as potential biomarkers against bacterial propagation in fish. Data from STRING-based functional network analyses indicate that fga is the most central protein. Our collective findings provide new insights into protein phosphorylation patterns, which may serve as effective indicators of A. salmonicida infection in Atlantic salmon.
DOI10.1038/s41598-019-38890-3
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2017YFD0701700] ; National Natural Science Foundation of China[31671376] ; Chinese State Key Projects for Basic Research (973 Program)[2015CB910700] ; Chinese State Key Projects for Basic Research (973 Program)[2014CBA02001] ; Beijing Nova Program[Z161100004916148] ; State Key Laboratory of Proteomics[SKLP-O201704] ; State Key Laboratory of Proteomics[SKLP-O201507] ; Innovation project[16CXZ027]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000458619600100
PublisherNATURE PUBLISHING GROUP
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155558
Collection实验海洋生物学重点实验室
Corresponding AuthorYang, Dung; Liu, Ying
Affiliation1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
4.Dalian Ocean Univ, Dalian, Peoples R China
5.Natl Ctr Prot Sci Beijing, State Key Lab Prote, Beijing Proteome Res Ctr, Beijing Inst Life, Beijing 102206, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,et al. Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida[J]. SCIENTIFIC REPORTS,2019,9:12.
APA Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,Li, Xian,Yang, Dung,&Liu, Ying.(2019).Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida.SCIENTIFIC REPORTS,9,12.
MLA Liu, Peng-fei,et al."Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida".SCIENTIFIC REPORTS 9(2019):12.
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