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Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis
Song Chengwen1,2; Liu Lei1; Hui Min1; Liu Yuan1,2; Liu Hourong1,3; Cui Zhaoxia1,2
2019
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume37Issue:1Pages:223-234
Corresponding AuthorCui Zhaoxia(zhxcui@qdio.ac.cn)
AbstractIn crustaceans, the male sexual differentiation and maintenance are specially regulated by androgenic gland (AG). However, little is known about the genes involved in the regulation process. RNA-Seq was performed on AG with ejaculatory duct (AG_ED) and ejaculatory duct (ED) as control in Eriocheir sinensis, one of the most important economic and fishery crabs with typically sex dimorphism. A total of 925 unigenes were identified as differentially expressed genes (DEGs) and the expression of nine genes randomly selected was confirmed by qRT-PCR. 667 unigenes were up-regulated in AG_ED, being supposed to be AG preferential genes. Among them, the full length of insulin-like androgenic gland factor (LAG) cDNA named as Es-IAG was obtained as a logo gene of AG, which together with the genes insulin-like receptor (IAW), and single insulin binding domain protein (SIBD), might constitute the sex regulation pathway. Several sex related genes were identified, and their function will have to be investigated. Also, the identification of juvenile hormone epoxide hydrolase I (JHEHI), ecdysteroid 22-hydroxylase (DIB) and ecdysone receptor (ECR) preliminarily clarified the molecular regulation mechanism of eyestalk-AG-testis axis, which plays important roles in molting and reproduction. The results will enhance our understanding for the molecular basis of the AG involved in male sex regulation in crabs.
Keywordandrogenic gland comparative transcriptome Eriocheir sinensis insulin-like androgenic gland factor (IAG) sex regulation
DOI10.1007/s00343-019-7254-6
Indexed BySCI
Language英语
Funding ProjectShandong Provincial Natural Science Foundation, China[ZR2014CP006] ; Independent Innovation Program of Qingdao[15-9-1-44-jch] ; Shandong Provincial Natural Science Foundation[ZR2017QD001] ; LIU Yuan
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000459304100020
PublisherSCIENCE PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155489
Collection实验海洋生物学重点实验室
Corresponding AuthorCui Zhaoxia
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Song Chengwen,Liu Lei,Hui Min,et al. Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019,37(1):223-234.
APA Song Chengwen,Liu Lei,Hui Min,Liu Yuan,Liu Hourong,&Cui Zhaoxia.(2019).Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,37(1),223-234.
MLA Song Chengwen,et al."Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 37.1(2019):223-234.
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