Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis

doi:10.1007/s00343-019-7254-6

IOCAS-IR > 实验海洋生物学重点实验室

	Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis
	Song Chengwen1,2 ; Liu Lei 1; Hui Min 1; Liu Yuan 1,2; Liu Hourong 1,3; Cui Zhaoxia 1,2
	2019
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
卷号	37 期号:1 页码:223-234
通讯作者	Cui Zhaoxia(zhxcui@qdio.ac.cn)
摘要	In crustaceans, the male sexual differentiation and maintenance are specially regulated by androgenic gland (AG). However, little is known about the genes involved in the regulation process. RNA-Seq was performed on AG with ejaculatory duct (AG_ED) and ejaculatory duct (ED) as control in Eriocheir sinensis, one of the most important economic and fishery crabs with typically sex dimorphism. A total of 925 unigenes were identified as differentially expressed genes (DEGs) and the expression of nine genes randomly selected was confirmed by qRT-PCR. 667 unigenes were up-regulated in AG_ED, being supposed to be AG preferential genes. Among them, the full length of insulin-like androgenic gland factor (LAG) cDNA named as Es-IAG was obtained as a logo gene of AG, which together with the genes insulin-like receptor (IAW), and single insulin binding domain protein (SIBD), might constitute the sex regulation pathway. Several sex related genes were identified, and their function will have to be investigated. Also, the identification of juvenile hormone epoxide hydrolase I (JHEHI), ecdysteroid 22-hydroxylase (DIB) and ecdysone receptor (ECR) preliminarily clarified the molecular regulation mechanism of eyestalk-AG-testis axis, which plays important roles in molting and reproduction. The results will enhance our understanding for the molecular basis of the AG involved in male sex regulation in crabs.
关键词	androgenic gland comparative transcriptome Eriocheir sinensis insulin-like androgenic gland factor (IAG) sex regulation
DOI	10.1007/s00343-019-7254-6
收录类别	SCI
语种	英语
资助项目	LIU Yuan ; Shandong Provincial Natural Science Foundation[ZR2017QD001] ; Independent Innovation Program of Qingdao[15-9-1-44-jch] ; Shandong Provincial Natural Science Foundation, China[ZR2014CP006] ; Shandong Provincial Natural Science Foundation, China[ZR2014CP006] ; Independent Innovation Program of Qingdao[15-9-1-44-jch] ; Shandong Provincial Natural Science Foundation[ZR2017QD001] ; LIU Yuan
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000459304100020
出版者	SCIENCE PRESS
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/155488
专题	实验海洋生物学重点实验室
通讯作者	Cui Zhaoxia
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Song Chengwen,Liu Lei,Hui Min,et al. Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019,37(1):223-234.
APA	Song Chengwen,Liu Lei,Hui Min,Liu Yuan,Liu Hourong,&Cui Zhaoxia.(2019).Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,37(1),223-234.
MLA	Song Chengwen,et al."Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 37.1(2019):223-234.