Examination of carbohydrate and lipid metabolic changes during Haematococcus pluvialis non-motile cell germination using transcriptome analysis

doi:10.1007/s10811-018-1524-0

IOCAS-IR > 实验海洋生物学重点实验室

	Examination of carbohydrate and lipid metabolic changes during Haematococcus pluvialis non-motile cell germination using transcriptome analysis
	Li, Qianqian1,2,3 ; Zhang, Litao 1,2,3; Liu, Jianguo 1,2,3
	2019-02-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	31 期号:1 页码:145-156
通讯作者	Liu, Jianguo(jgliu@qdio.ac.cn)
摘要	During the complex Haematococcus pluvialis life cycle, germination from non-motile cells to motile cells is the key stage for cell recovery from resting spores. Three phases can be recognized: first, repeated mitotic events; next, cytokinesis to form the zoospore; and finally, a fast release of motile cells. After HiSeq 2000 sequencing of RNA collected at four time points during non-motile cell germination, a total of 2202 differentially expressed genes (DEGs) were identified. In the expression profiles, there is a consistent increase in the expression level of -amylase and ACAA1 in the entire process, indicating starch and lipid mobilization. In phase 1, two down-regulated genes (fructose-1, 6-bisphosphate aldolase (FBA) and pyruvate kinase (PK)) of glycolysis limited the provision of acetyl-CoA for the TCA cycle, which could be compensated by fatty acid degradation (up-regulation of ACAA1) in the glyoxysome. In phase 2, nine and eight up-regulated enzymes of carbohydrate (glycolysis, TCA cycle, and pentose phosphate pathway) and lipid (fatty acid synthesis and degradation) metabolism, respectively, would increase the metabolic rate and come into a balance between production and consumption of starch and lipid. Till phase 3, the expression of the vast majority of carbohydrate metabolism-related DEGs remained high, while lipid metabolism did not. This suggested that the carbon flux centered on carbohydrate metabolism in this phase. In addition, several isozymes of FBA, GAPDH, PK, and so on were separated by SMART analysis and are postulated to serve different actions during H. pluvialis non-motile cell germination.
关键词	Haematococcus pluvialis Non-motile cell Motile cell Germination Metabolic change
DOI	10.1007/s10811-018-1524-0
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31572639] ; National Natural Science Foundation of China[31702366] ; National Natural Science Foundation of China[31702366] ; National Natural Science Foundation of China[31572639]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:000458698200014
出版者	SPRINGER
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/155421
专题	实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Yunnan Alphy Biotech Co Ltd, Natl Local Joint Engn Res Ctr Haematococcus Pluvi, Chuxiong 675012, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Qianqian,Zhang, Litao,Liu, Jianguo. Examination of carbohydrate and lipid metabolic changes during Haematococcus pluvialis non-motile cell germination using transcriptome analysis[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(1):145-156.
APA	Li, Qianqian,Zhang, Litao,&Liu, Jianguo.(2019).Examination of carbohydrate and lipid metabolic changes during Haematococcus pluvialis non-motile cell germination using transcriptome analysis.JOURNAL OF APPLIED PHYCOLOGY,31(1),145-156.
MLA	Li, Qianqian,et al."Examination of carbohydrate and lipid metabolic changes during Haematococcus pluvialis non-motile cell germination using transcriptome analysis".JOURNAL OF APPLIED PHYCOLOGY 31.1(2019):145-156.