Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida

doi:10.1007/s10811-018-1576-1

IOCAS-IR > 实验海洋生物学重点实验室

	Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida
	Li, Jing1,2 ; Huang, Haishu 1,2,3; Shan, Tifeng 1,2; Pang, Shaojun 1,2
	2019-02-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	31 期号:1 页码:787-793
通讯作者	Pang, Shaojun(sjpang@ms.qdio.ac.cn)
摘要	Quantitative real-time PCR (qRT-PCR) has become widely used method for detecting gene expression of an organism at different developmental stages or under various stressful environmental conditions. Finding reference genes is a prerequisite in this process. In this research, we investigated the expression stability of eight candidate genes in the brown alga Undaria pinnatifida, including EF1 (elongation factor 1-alpha), eEF1 (eukaryotic elongation factor-1 B beta), RPL35 (60S ribosomal protein L35-3), GAPDH (glyceraldehyde-3-phosphatedehydrogenase), RPS17 (40S ribosomal protein S17), LHCP (light-harvesting complex protein), ribH (6,7-dimethyl-8-ribityllumazine synthase), and ilvC (ketol-acid reductoisomerase). The haploid male and female gametophytes at different stages, as well as the diploid sporophytes treated with different abiotic factors were analyzed by geNorm and NormFinder software. It was found that eEF1 and ribH could be used as the most stable reference genes in studies of gender or developmental gene expression in the gametophytes. As for the detection of gene expression in the sporophytes, eEF1 and ribH were recommended as the most suitable reference genes for irradiance treatment, eEF1 and RPL35 for temperature treatment, while EF1 and LHCP for nutrition treatment. These results will enable us to go further for quantifying gene expression study at transcript level in U. pinnatifida.
关键词	Reference genes qRT-PCR geNorm NormFinder Phaeophyta Undaria pinnatifida
DOI	10.1007/s10811-018-1576-1
收录类别	SCI
语种	英语
资助项目	Huiquan Scholar Program of Institute of Oceanology, Chinese Academy of Sciences ; Taishan Scholar Program of Shandong Province ; National Infrastructure of Fishery Germplasm Resource[2017DKA30470] ; National Key Technology Support Program[2015BAD13B05] ; China Agriculture Research System[CARS-50] ; Sino-German Science Center[GZ 1080] ; National Natural Science Foundation of China[41676128] ; National Natural Science Foundation of China[31702365] ; National Natural Science Foundation of China[41476141] ; National Natural Science Foundation of China[41476141] ; National Natural Science Foundation of China[31702365] ; National Natural Science Foundation of China[41676128] ; Sino-German Science Center[GZ 1080] ; China Agriculture Research System[CARS-50] ; National Key Technology Support Program[2015BAD13B05] ; National Infrastructure of Fishery Germplasm Resource[2017DKA30470] ; Taishan Scholar Program of Shandong Province ; Huiquan Scholar Program of Institute of Oceanology, Chinese Academy of Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:000458698200069
出版者	SPRINGER
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/155412
专题	实验海洋生物学重点实验室
通讯作者	Pang, Shaojun
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Jing,Huang, Haishu,Shan, Tifeng,et al. Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(1):787-793.
APA	Li, Jing,Huang, Haishu,Shan, Tifeng,&Pang, Shaojun.(2019).Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida.JOURNAL OF APPLIED PHYCOLOGY,31(1),787-793.
MLA	Li, Jing,et al."Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida".JOURNAL OF APPLIED PHYCOLOGY 31.1(2019):787-793.