Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida | |
Li, Jing1,2; Huang, Haishu1,2,3; Shan, Tifeng1,2; Pang, Shaojun1,2 | |
2019-02-01 | |
发表期刊 | JOURNAL OF APPLIED PHYCOLOGY |
ISSN | 0921-8971 |
卷号 | 31期号:1页码:787-793 |
通讯作者 | Pang, Shaojun(sjpang@ms.qdio.ac.cn) |
摘要 | Quantitative real-time PCR (qRT-PCR) has become widely used method for detecting gene expression of an organism at different developmental stages or under various stressful environmental conditions. Finding reference genes is a prerequisite in this process. In this research, we investigated the expression stability of eight candidate genes in the brown alga Undaria pinnatifida, including EF1 (elongation factor 1-alpha), eEF1 (eukaryotic elongation factor-1 B beta), RPL35 (60S ribosomal protein L35-3), GAPDH (glyceraldehyde-3-phosphatedehydrogenase), RPS17 (40S ribosomal protein S17), LHCP (light-harvesting complex protein), ribH (6,7-dimethyl-8-ribityllumazine synthase), and ilvC (ketol-acid reductoisomerase). The haploid male and female gametophytes at different stages, as well as the diploid sporophytes treated with different abiotic factors were analyzed by geNorm and NormFinder software. It was found that eEF1 and ribH could be used as the most stable reference genes in studies of gender or developmental gene expression in the gametophytes. As for the detection of gene expression in the sporophytes, eEF1 and ribH were recommended as the most suitable reference genes for irradiance treatment, eEF1 and RPL35 for temperature treatment, while EF1 and LHCP for nutrition treatment. These results will enable us to go further for quantifying gene expression study at transcript level in U. pinnatifida. |
关键词 | Reference genes qRT-PCR geNorm NormFinder Phaeophyta Undaria pinnatifida |
DOI | 10.1007/s10811-018-1576-1 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Huiquan Scholar Program of Institute of Oceanology, Chinese Academy of Sciences ; Taishan Scholar Program of Shandong Province ; National Infrastructure of Fishery Germplasm Resource[2017DKA30470] ; National Key Technology Support Program[2015BAD13B05] ; China Agriculture Research System[CARS-50] ; Sino-German Science Center[GZ 1080] ; National Natural Science Foundation of China[41676128] ; National Natural Science Foundation of China[31702365] ; National Natural Science Foundation of China[41476141] ; National Natural Science Foundation of China[41476141] ; National Natural Science Foundation of China[31702365] ; National Natural Science Foundation of China[41676128] ; Sino-German Science Center[GZ 1080] ; China Agriculture Research System[CARS-50] ; National Key Technology Support Program[2015BAD13B05] ; National Infrastructure of Fishery Germplasm Resource[2017DKA30470] ; Taishan Scholar Program of Shandong Province ; Huiquan Scholar Program of Institute of Oceanology, Chinese Academy of Sciences |
WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
WOS类目 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
WOS记录号 | WOS:000458698200069 |
出版者 | SPRINGER |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/155412 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Pang, Shaojun |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Li, Jing,Huang, Haishu,Shan, Tifeng,et al. Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(1):787-793. |
APA | Li, Jing,Huang, Haishu,Shan, Tifeng,&Pang, Shaojun.(2019).Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida.JOURNAL OF APPLIED PHYCOLOGY,31(1),787-793. |
MLA | Li, Jing,et al."Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida".JOURNAL OF APPLIED PHYCOLOGY 31.1(2019):787-793. |
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