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Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida
Li, Jing1,2; Huang, Haishu1,2,3; Shan, Tifeng1,2; Pang, Shaojun1,2
2019-02-01
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
Volume31Issue:1Pages:787-793
Corresponding AuthorPang, Shaojun(sjpang@ms.qdio.ac.cn)
AbstractQuantitative real-time PCR (qRT-PCR) has become widely used method for detecting gene expression of an organism at different developmental stages or under various stressful environmental conditions. Finding reference genes is a prerequisite in this process. In this research, we investigated the expression stability of eight candidate genes in the brown alga Undaria pinnatifida, including EF1 (elongation factor 1-alpha), eEF1 (eukaryotic elongation factor-1 B beta), RPL35 (60S ribosomal protein L35-3), GAPDH (glyceraldehyde-3-phosphatedehydrogenase), RPS17 (40S ribosomal protein S17), LHCP (light-harvesting complex protein), ribH (6,7-dimethyl-8-ribityllumazine synthase), and ilvC (ketol-acid reductoisomerase). The haploid male and female gametophytes at different stages, as well as the diploid sporophytes treated with different abiotic factors were analyzed by geNorm and NormFinder software. It was found that eEF1 and ribH could be used as the most stable reference genes in studies of gender or developmental gene expression in the gametophytes. As for the detection of gene expression in the sporophytes, eEF1 and ribH were recommended as the most suitable reference genes for irradiance treatment, eEF1 and RPL35 for temperature treatment, while EF1 and LHCP for nutrition treatment. These results will enable us to go further for quantifying gene expression study at transcript level in U. pinnatifida.
KeywordReference genes qRT-PCR geNorm NormFinder Phaeophyta Undaria pinnatifida
DOI10.1007/s10811-018-1576-1
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41476141] ; National Natural Science Foundation of China[31702365] ; National Natural Science Foundation of China[41676128] ; Sino-German Science Center[GZ 1080] ; China Agriculture Research System[CARS-50] ; National Key Technology Support Program[2015BAD13B05] ; National Infrastructure of Fishery Germplasm Resource[2017DKA30470] ; Taishan Scholar Program of Shandong Province ; Huiquan Scholar Program of Institute of Oceanology, Chinese Academy of Sciences
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000458698200069
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155412
Collection实验海洋生物学重点实验室
Corresponding AuthorPang, Shaojun
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Jing,Huang, Haishu,Shan, Tifeng,et al. Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(1):787-793.
APA Li, Jing,Huang, Haishu,Shan, Tifeng,&Pang, Shaojun.(2019).Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida.JOURNAL OF APPLIED PHYCOLOGY,31(1),787-793.
MLA Li, Jing,et al."Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida".JOURNAL OF APPLIED PHYCOLOGY 31.1(2019):787-793.
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