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Understanding the roles of surface proteins in regulation of Lactobacillus pentosus HC-2 to immune response and bacterial diversity in midgut of Litopenaeus vannamei
Du, Yang1,2; Zhou, Shuhong2,3; Liu, Mei1,2; Wang, Baojie1,2; Jiang, Keyong1,2; Fang, Han2,3; Wang, Lei1,2,4
2019-03-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号86页码:1194-1206
通讯作者Wang, Lei(wanglei@qdio.ac.cn)
摘要The interactions of microbiota in the intestines play an important role in promoting or maintaining the health of hosts. The present study aim to investigate the effects of the surface proteins of Lactobacillus pentosus HC-2 on the immune response and the bacterial composition of Litopenaeus vannamei, thus, the immune -related genes, surface condition, HC-2 numbers and the bacteria diversity in midgut were explored after shrimp feeding the normal HC-2 and 5 M - lithium chloride (LiCI) treated HC-2 for four weeks. Obvious improvements in the intestinal surface were observed in R group than the control group and L group. qPCR analysis demonstrated that the selected immune -related genes of lysozyme, proPO, LGBP, PEN-3 alpha, crustin, and lvLec were significantly up-regulated in group R than in group L Meanwhile, in the challenge test, shrimp in R group received 72% relative percent survival, which was significantly higher than the L group (RPS = 9%). The bacteria composition analysis showed that the abundance of Proteobacteria were significantly higher in group R and L than in group C, and the Bacteroidetes were significantly higher in group C than in group R and L, whereas the numbers of Chloroflexi were significantly higher in group R than in group C and L. The bacterial community difference analysis revealed that the harmful bacteria such as genus of Vibrio, Tenacibaculu and Thalassobius were decreased and the beneficial bacterium as Ruegeria and Lactobacillus were increased in R group, whereas this phenomenon were not found in L group. Taken together, above results indicating that the surface proteins were indispensable for L. pentosus HC-2 adhesion and colonization in shrimp intestines to improve intestine condition, enhance immune response, competitively exclude the pathogens, and promote the beneficial bacteria growth to protect the shrimp from pathogens infection. The findings in this work will help to promote the understanding of the roles of probiotics in shrimp intestines displaying probiotic-function by regulating the intestinal bacteria.
关键词Litopenaeus vannamei Lactobacillus pentosus HC-2 Surface proteins Immune response Gut bacteria diversity
DOI10.1016/j.fsi.2018.12.073
收录类别SCI
语种英语
资助项目China Postdoctoral Science Foundation[2018M632736] ; Scientific and technological development fund project of Shinan district of Qingdao city[2018-4-001-ZH] ; National Natural Science Foundation of China[31802309] ; National Natural Science Foundation of China[31802309] ; Scientific and technological development fund project of Shinan district of Qingdao city[2018-4-001-ZH] ; China Postdoctoral Science Foundation[2018M632736]
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号WOS:000459528400132
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/155394
专题实验海洋生物学重点实验室
通讯作者Wang, Lei
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
4.Chinese Acad Sci, CAS Ctr Ocean Mega Sci, Qingdao 266400, Peoples R China
第一作者单位中国科学院海洋研究所
通讯作者单位中国科学院海洋研究所
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Du, Yang,Zhou, Shuhong,Liu, Mei,et al. Understanding the roles of surface proteins in regulation of Lactobacillus pentosus HC-2 to immune response and bacterial diversity in midgut of Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2019,86:1194-1206.
APA Du, Yang.,Zhou, Shuhong.,Liu, Mei.,Wang, Baojie.,Jiang, Keyong.,...&Wang, Lei.(2019).Understanding the roles of surface proteins in regulation of Lactobacillus pentosus HC-2 to immune response and bacterial diversity in midgut of Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,86,1194-1206.
MLA Du, Yang,et al."Understanding the roles of surface proteins in regulation of Lactobacillus pentosus HC-2 to immune response and bacterial diversity in midgut of Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 86(2019):1194-1206.
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