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Unraveling the molecular mechanism of the response to changing ambient phosphorus in the dinoflagellate Alexandrium catenella with quantitative proteomics
Zhang, Shu-Feng1; Chen, Ying1; Xie, Zhang-Xian1; Zhang, Hao1; Lin, Lin1; Wang, Da-Zhi1,2
2019-03-30
Source PublicationJOURNAL OF PROTEOMICS
ISSN1874-3919
Volume196Pages:141-149
Corresponding AuthorWang, Da-Zhi(dzwang@xmu.edu.cn)
AbstractPhosphorus (P) is a key macronutrient limiting cell growth and bloom formation of marine dinoflagellates. Physiological responses to changing ambient P have been investigated in dinoflagellates; however, the molecular mechanisms behind these responses remain limited. Here, we compared the protein expression profiles of a marine dinoflagellate Alexandrium catenella grown in inorganic P-replete, P-deficient, and inorganic- and organic-P resupplied conditions using an iTRAQ-based quantitative proteomic approach. P deficiency inhibited cell growth and enhanced alkaline phosphatase activity (APA) but had no effect on photosynthetic efficiency. After P resupply, the P-deficient cells recovered growth rapidly and APA decreased. Proteins involved in sphingolipid metabolism, organic P utilization, starch and sucrose metabolism, and photosynthesis were up-regulated in the P-deficient cells, while proteins associated with protein synthesis, nutrient assimilation and energy metabolism were down-regulated. The responses of the P-deficient A. catenella to the resupply of organic and inorganic P presented significant differences: more biological processes were enhanced in the organic P-resupplied cells than those in the inorganic P-resupplied cells; A. catenella might directly utilize G-6-P for nucleic acid synthesis through the pentose phosphate pathway. Our results indicate that A. catenella has evolved diverse adaptive strategies to ambient P deficiency and specific mechanisms to utilize dissolved organic P, which might be an important reason resulting in A. catenella bloom in the low inorganic P environment. Biological significance: The ability of marine dinoflagellates to utilize different phosphorus (P) species and adapt to ambient P deficiency determines their success in the ocean. In this study, we investigated the response mechanisms of a dinoflagellate Alexandrium catenella to ambient P deficiency, and resupply of inorganic- and organic-P at the proteome level. Our results indicated that A. catenella initiated multiple adaptive strategies to ambient P deficiency, e.g. utilizing nonphospholipids and glycosphingolipids instead of phospholipids, enhancing expression of acid phosphatase to utilize organic P, and reallocating intracellular energy. Proteome responses of the P-deficient A. catenella to resupply of inorganic- and organic-P differed significantly, indicating different utilization pathways of inorganic and organic P, A. catenella might directly utilize low molecular weight organic P, such as G-6-P as both P and carbon sources.
KeywordMarine dinoflagellates Harmful algal blooms Alexandrium catenella Phosphorus Quantitative proteomics
DOI10.1016/j.jprot.2018.11.004
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2017YFC1404300] ; National Natural Science Foundation of China[41230961] ; National Natural Science Foundation of China[41706131] ; National Natural Science Foundation of China[41425021] ; 'Ten Thousand Talents Program' for leading talents in science and technological innovation
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000460716800013
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155271
Collection海洋生态与环境科学重点实验室
Corresponding AuthorWang, Da-Zhi
Affiliation1.Xiamen Univ, Coll Environm & Ecol, State Key Lab Marine Environm Sci, Xiamen 361102, Fujian, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Shu-Feng,Chen, Ying,Xie, Zhang-Xian,et al. Unraveling the molecular mechanism of the response to changing ambient phosphorus in the dinoflagellate Alexandrium catenella with quantitative proteomics[J]. JOURNAL OF PROTEOMICS,2019,196:141-149.
APA Zhang, Shu-Feng,Chen, Ying,Xie, Zhang-Xian,Zhang, Hao,Lin, Lin,&Wang, Da-Zhi.(2019).Unraveling the molecular mechanism of the response to changing ambient phosphorus in the dinoflagellate Alexandrium catenella with quantitative proteomics.JOURNAL OF PROTEOMICS,196,141-149.
MLA Zhang, Shu-Feng,et al."Unraveling the molecular mechanism of the response to changing ambient phosphorus in the dinoflagellate Alexandrium catenella with quantitative proteomics".JOURNAL OF PROTEOMICS 196(2019):141-149.
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