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Molecular mechanism of inbreeding effects based on RNA-Seq analysis of the adductor muscle of bay scallop (Argopecten irradians)
Wang, Yu1,3,6; Zhang, Shoudu4; Zhang, Guofan1,2,5; Cong, Rihao1,2,5; Li, Li1,5
2019-02-01
Source PublicationACTA OCEANOLOGICA SINICA
ISSN0253-505X
Volume38Issue:2Pages:44-51
Corresponding AuthorCong, Rihao(rhcong@qdio.ac.cn) ; Li, Li(lili@qdio.ac.cn)
AbstractUnderstanding the effects of inbreeding is critical for the long-term viability of shellfish breeding and for understanding inbreeding depression (ID) of the offspring of selfed individuals and full-sib crosses. The bay scallop (Argopecten irradians) is a simultaneous hermaphroditic marine bivalve with distinct male and female gonads and is a species with external fertilization. It is a canonical model invertebrate for analysis of the genetics of ID and evolution of outcrossing mating system. ID in bivalves is well documented with regard to the phenotype and molecular markers aspects, but little is known on how inbreeding affects genome-wide gene expression. Here, we compare the gene expression profiles of adductor muscle of A. irradians lines inbred to F=0.5 with those of its counterpart outbred lines which are originated from the same paternal individual. Inbred and outbred individuals have some differences in growth traits. The shell length and the total weight of inbred scallops are lower than the counterpart of outbred lines. We found that 1 175 genes were up-regulated while 1 390 were downregulated in inbred lines. Regarding components, differentially expressed genes (DEGs) were concentrated on sarcomeres, contractile filaments, and cell membranes. Among others, whereas regarding their function, the Gene Ontology (GO) was concentrated on transferase, phosphotransferase, peptidase, and others. The DEGs-enriched metabolic pathways mainly belonged to the oxidative phosphorylation pathway and apoptosis pathway. We used another two families to verify our bioinformatic results. The selfed and the outbred family originated from different ancestors, but the selfed family showed obvious ID. By using real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), we verified that DEGs with inbreeding are involved in the oxidative phosphorylation and cell apoptosis pathways, suggesting that inbreeding may affect energy metabolism and homeostasis.
KeywordArgopecten irradians inbreeding depression differentially expressed genes transcriptome
DOI10.1007/s13131-019-1351-4
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[30800842] ; National Natural Science Foundation of China[31502164] ; Program of the Chinese Academy of Science[ZKCX2-211] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49]
WOS Research AreaOceanography
WOS SubjectOceanography
WOS IDWOS:000460726200005
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155257
Collection实验海洋生物学重点实验室
Corresponding AuthorCong, Rihao; Li, Li
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Tianjin Bohai Sea Fisheries Res Inst, Tianjin 300457, Peoples R China
4.Natl Oceanog Ctr, Qingdao 266071, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
6.Tianjin Ocean Ranch Tech Engn Ctr, Tianjin 300457, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Yu,Zhang, Shoudu,Zhang, Guofan,et al. Molecular mechanism of inbreeding effects based on RNA-Seq analysis of the adductor muscle of bay scallop (Argopecten irradians)[J]. ACTA OCEANOLOGICA SINICA,2019,38(2):44-51.
APA Wang, Yu,Zhang, Shoudu,Zhang, Guofan,Cong, Rihao,&Li, Li.(2019).Molecular mechanism of inbreeding effects based on RNA-Seq analysis of the adductor muscle of bay scallop (Argopecten irradians).ACTA OCEANOLOGICA SINICA,38(2),44-51.
MLA Wang, Yu,et al."Molecular mechanism of inbreeding effects based on RNA-Seq analysis of the adductor muscle of bay scallop (Argopecten irradians)".ACTA OCEANOLOGICA SINICA 38.2(2019):44-51.
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