IOCAS-IR  > 实验海洋生物学重点实验室
The transcriptional response of the Pacific oyster Crassostrea gigas under simultaneous bacterial and heat stresses
Zhang, Huan1,4; Wang, Hao1,4; Chen, Hao1,4; Wang, Mengqiang1,2; Zhou, Zhi2; Qiu, Limei1,2; Wang, Lingling3; Song, Linsheng3
2019-05-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume94Pages:1-10
Corresponding AuthorWang, Hao() ; Song, Linsheng(lshsong@dlou.edu.cn)
AbstractBacterial infection and heat stress are considered as two major environmental threats for the aquaculture industry of oyster Crassostrea gigas. In the present study, the expression profiles of mRNA transcripts in the hemocytes of oysters under bacterial challenge and heat stress were examined by next-generation sequencing. There were 21,095, 21,957 and 21,141 transcripts identified in the hemocytes of oysters from three groups, respectively, including control group (designated as Con group), Vibrio splendidus challenge group (Bac group), and bacterial and heat stress combined treatment group (BacHeat group). There were 4610, 5093 and 5149 differentially expressed transcripts (DTs) in the three pairwise comparisons Con/Bac, Con/BacHeat and Bac/BacHeat, respectively. The main enriched GO terms in biological process category of the DTs included the metabolic processes, cellular process, response to stimulus and immune system process. The expression patterns of DTs involved in pattern recognition, immune signal transduction and energy metabolic indicated that the immune response to bacterial challenge was disturbed under acute heat stress, which was also confirmed by quantitative real-time PCR. The neuroendocrine immunomodulation, especially the catecholaminergic regulation, played indispensable roles in stress response. The total energy reserves as well as cellular energy allocation (CEA) in hepatopancreas of oysters decreased remarkably especially in BacHeat group, while the energy consumption generally increased, suggesting that the immune defense against the simultaneous stimulation of pathogen and heat stress imposed greater costs on oyster's energy expenditure than a single stressor. These results above indicated that, the heat stress disturbed the normal expression of genes involved in immune response and energy metabolism, accelerated energy consumption and broke the metabolic balance, leading to a decline in resilience to infection and mass mortality of oyster in summer.
KeywordCrassostrea gigas Transcriptome analysis Vibrio splendidus Immune challenge Heat stress Energy metabolic
DOI10.1016/j.dci.2019.01.006
Indexed BySCI
Language英语
Funding ProjectNational Science Foundation of China[41476145,31530069] ; [2018YFD0900606]
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000460826100001
PublisherELSEVIER SCI LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155244
Collection实验海洋生物学重点实验室
深海极端环境与生命过程研究中心
Corresponding AuthorWang, Hao; Song, Linsheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, 52 Heishijiao St Dalian, Dalian 116023, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
First Author AffilicationChinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res
Corresponding Author AffilicationChinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res
Recommended Citation
GB/T 7714
Zhang, Huan,Wang, Hao,Chen, Hao,et al. The transcriptional response of the Pacific oyster Crassostrea gigas under simultaneous bacterial and heat stresses[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,94:1-10.
APA Zhang, Huan.,Wang, Hao.,Chen, Hao.,Wang, Mengqiang.,Zhou, Zhi.,...&Song, Linsheng.(2019).The transcriptional response of the Pacific oyster Crassostrea gigas under simultaneous bacterial and heat stresses.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,94,1-10.
MLA Zhang, Huan,et al."The transcriptional response of the Pacific oyster Crassostrea gigas under simultaneous bacterial and heat stresses".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 94(2019):1-10.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Huan]'s Articles
[Wang, Hao]'s Articles
[Chen, Hao]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Huan]'s Articles
[Wang, Hao]'s Articles
[Chen, Hao]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Huan]'s Articles
[Wang, Hao]'s Articles
[Chen, Hao]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.