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Muscle fibre type composition in the lateral muscle of olive flounder Paralichthys olivaceus
Jiao, Shuang1,2,3; Tan, Xungang1,2,3; Sui, Yulei1,2,3,4; You, Feng1,2,3
2019
Source PublicationACTA HISTOCHEMICA
ISSN0065-1281
Volume121Issue:1Pages:1-6
Corresponding AuthorTan, Xungang(tanx@qdio.ac.cn)
AbstractIn this paper, a combined-method study has been made on the lateral muscle of the teleost olive flounder Paralichthys olivaceus in just-hatched and adult stages. In just-hatched stage, both slow and fast muscle fibres were detected: (1) in situ hybridization analysis indicated that slow and fast myosin heavy chain genes were specifically expressed in the superficial and deep part of the myotomal muscle, respectively; (2) immunohistochemistry analysis showed that fibres in the deep part reacted with anti-fast myosin antibody F310; (3) western blot analysis detected a weak expression of slow myosin and a strong expression of fast myosin. In adult stage, the slow and fast muscle fibres had their own distribution characteristics: (1) hematoxylin/eosin staining showed the histological characteristics of the muscle fibre composition; (2) histochemical observations showed that the deep muscle fibres, and some fibres near the epidermis, contain alkali-stable myofibrillar ATPase activity; (3) immunohistochemistry analysis indicated that all the deep muscle fibres reacted with F310 antibody and some fibres in the superficial layer of muscle also reacted with F310; (4) western blot analysis showed that fast myosin was expressed both in the blended muscles (the mix of superficial and deep muscles) and deep muscles, while slow myosin was mainly expressed in the blended muscles. These findings suggested that both slow and fast muscle fibres existed in the musculature of the olive flounder in just-hatched and adult stages. Notably, the adult fast fibres also exist in the superficial layer of the muscle.
KeywordOlive flounder Muscle fibre Fast muscle Slow muscle
DOI10.1016/j.acthis.2018.10.002
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31502146] ; National Natural Science Foundation of China[31672636] ; National Flatfish Industry System Construction Programme of China[nycytx-50] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Infrastructure of Fishery Germplasm Resource[2017DKA30470]
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000461000600001
PublisherELSEVIER GMBH
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155235
Collection实验海洋生物学重点实验室
Corresponding AuthorTan, Xungang
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jiao, Shuang,Tan, Xungang,Sui, Yulei,et al. Muscle fibre type composition in the lateral muscle of olive flounder Paralichthys olivaceus[J]. ACTA HISTOCHEMICA,2019,121(1):1-6.
APA Jiao, Shuang,Tan, Xungang,Sui, Yulei,&You, Feng.(2019).Muscle fibre type composition in the lateral muscle of olive flounder Paralichthys olivaceus.ACTA HISTOCHEMICA,121(1),1-6.
MLA Jiao, Shuang,et al."Muscle fibre type composition in the lateral muscle of olive flounder Paralichthys olivaceus".ACTA HISTOCHEMICA 121.1(2019):1-6.
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