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High temperature increases the gsdf expression in masculinization of genetically female Japanese flounder (Paralichthys olivaceus)
Yang, Yang1,2,3,5; Liu, Qinghua1,2,5; Xiao, Yongshuang1,2,5; Xu, Shihong1,2,5; Wang, Xueying1,2,5; Yang, Jingkun2; Song, Zongcheng4; You, Feng1,2,5; Li, Jun1,2,5
2019-04-01
Source PublicationGENERAL AND COMPARATIVE ENDOCRINOLOGY
ISSN0016-6480
Volume274Pages:17-25
Corresponding AuthorLiu, Qinghua(qinghualiu@qdio.ac.cn)
AbstractIn teleosts, sex is plastic and is influenced by environmental factors. Elevated temperatures have masculinizing effects on the phenotypic sex of certain sensitive species. In this study, we reared genetic XX Japanese flounder at a high temperature (27.5 +/- 0.5 degrees C) and obtained a population of sex-reversal XX males (male ratio, 95.24%). We comparatively analyzed the dynamic characteristics of germ cells and gsdf (gonadal soma-derived factor) expression during sexual differentiation for the experimental (27.5 +/- 0.5 degrees C) and control (18 +/- 0.5 degrees C) groups. The results revealed that the germ cell proliferation inhibited and gsdf expression up-regulated in the experimental group, and the gsdf mRNA and proteins expressed in somatic cells that had direct contact with germline stem cells (with Nanos 2 protein expression) including spermatogonia and oogonia by ISH (in situ hybridization) and IHC (immunohistochemistry). In addition, we also overexpressed the gsdf in XX flounders, and the germ cell number of XX flounders bearing gsdf gene significantly decreased and sometimes disappeared completely, which was consistent with the results from high-temperature induction. Therefore, based on all the results, we speculated that the high expression of gsdf might inhibit germ cell proliferation during sex differentiation, and eventually cause sex reversal in the high-temperature induced masculinization of XX Japanese flounder.
KeywordHigh temperature Sex reversal Gsdf Japanese flounder Germline stem cell
DOI10.1016/j.ygcen.2018.12.012
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; China Agriculture Research System[CARS -47] ; China Postdoctoral Science Foundation[2016M592259] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060]
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000460849000003
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155233
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Qinghua
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Weihai Shenghang Aquat Product Sci & Technol Co L, Weihai 264200, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nankai Rd, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang, Yang,Liu, Qinghua,Xiao, Yongshuang,et al. High temperature increases the gsdf expression in masculinization of genetically female Japanese flounder (Paralichthys olivaceus)[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2019,274:17-25.
APA Yang, Yang.,Liu, Qinghua.,Xiao, Yongshuang.,Xu, Shihong.,Wang, Xueying.,...&Li, Jun.(2019).High temperature increases the gsdf expression in masculinization of genetically female Japanese flounder (Paralichthys olivaceus).GENERAL AND COMPARATIVE ENDOCRINOLOGY,274,17-25.
MLA Yang, Yang,et al."High temperature increases the gsdf expression in masculinization of genetically female Japanese flounder (Paralichthys olivaceus)".GENERAL AND COMPARATIVE ENDOCRINOLOGY 274(2019):17-25.
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