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Insights into individual variations in nematocyst venoms from the giant jellyfish Nemopilema nomurai in the Yellow Sea
Yue, Yang1,3; Yu, Huahua1,2,3; Li, Rongfeng1,2,3; Liu, Song1,2,3; Xing, Ronge1,2,3; Li, Pengcheng1,2,3
2019-03-04
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume9Pages:13
Corresponding AuthorYu, Huahua(yuhuahua@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
AbstractThe giant jellyfish, Nemopilema nomurai, is widely distributed from the Eastern China Sea to the northern part of the Yellow Sea and has resulted in numerous hospitalizations in coastal areas of China, especially in Northern China. Our previous studies have revealed sting-related proteins in the venom of the jellyfish N. nomurai by using experimental and omics-based approaches; however, the variable symptoms of patients who have been stung by N. nomurai are not fully understood. This limited knowledge led to an examination of whether intraspecific variations occur in the venom of different N. nomurai. In the present study, 13 specimens of N. nomurai were collected from the Yellow Sea, and their venom was characterized by profiling differences in biochemical properties and biological activities. SDS-PAGE analysis presented recognizable differences in the number, intensity and presence of some protein bands. Moreover, enzymatic assays revealed considerable quantitative variations in metalloproteinase activity and PLA2-like activity. In particular, zymography assays of proteases demonstrated the general presence of abundant metalloproteinases in jellyfish nematocyst venom; however, the catalytic activities varied greatly among some specific metalloproteinases in the 28-46 kDa or 57-83 kDa range. Hemolytic assays using sheep erythrocytes suggested a predominant variance in the toxicities of different individual jellyfish venoms, with the difference between the most hemolytic and the least hemolytic venom as large as 77-fold. The current data suggested remarkable variations in the nematocyst venoms of individual N. nomurai jellyfish. These observations will provide a new understanding of the clinical manifestations induced by N. nomurai jellyfish stings and will therefore have important implications for preventing and treating jellyfish envenomations.
DOI10.1038/s41598-019-40109-4
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41776163] ; National Natural Science Foundation of China[41876164] ; National Natural Science Foundation of China[41376004] ; National Natural Science Foundation of China[41406152] ; National Key RD Plan[2017YFE0111100-04] ; Key Research and Development Plan of Shandong Province[2017GHY215003] ; Qingdao Science and Technology Project[17-3-3-21-nsh]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000460123600033
PublisherNATURE PUBLISHING GROUP
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155230
Collection实验海洋生物学重点实验室
Corresponding AuthorYu, Huahua; Li, Pengcheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Biol Prod, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yue, Yang,Yu, Huahua,Li, Rongfeng,et al. Insights into individual variations in nematocyst venoms from the giant jellyfish Nemopilema nomurai in the Yellow Sea[J]. SCIENTIFIC REPORTS,2019,9:13.
APA Yue, Yang,Yu, Huahua,Li, Rongfeng,Liu, Song,Xing, Ronge,&Li, Pengcheng.(2019).Insights into individual variations in nematocyst venoms from the giant jellyfish Nemopilema nomurai in the Yellow Sea.SCIENTIFIC REPORTS,9,13.
MLA Yue, Yang,et al."Insights into individual variations in nematocyst venoms from the giant jellyfish Nemopilema nomurai in the Yellow Sea".SCIENTIFIC REPORTS 9(2019):13.
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