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扇贝营养需求与代用饲料研究
王健
学位类型博士
1999
学位授予单位中国科学院海洋研究所
学位授予地点中国科学院海洋研究所
关键词栉孔扇贝 黑西哥湾扇贝 饥饿 蛋白源 营养需求 配合饲料
摘要营养学是生物学研究的重要组成部分。对养殖对象营养需求的深入研究是未来养殖业持续健康发展的重要保证。滤食性贝类营养学研究起步较晚,与鱼、虾营养学相比也有不小的差距。本论文在揭示饥饿对栉孔扇贝肥满度、营养组成和代谢影响的基础上,着重研究了不同蛋白源对栉孔扇贝生长的影响和黑西哥湾扇贝对主要营养成分的需求。实验部分 I:目前,贝类营养学研究通常是通过强化某一种工某几种营养来进行的,而在饥饿这一特殊条件下贝类的生理活动情况却少见报导。本实验研究了饥饿对扇贝生命活动的影响,从这一特殊的角度研究了扇贝对各种营养组分的利用情况,同时为揭示近年来栉孔扇贝大规模死亡的原因和机制提供参考数据。实验结果表明,饥饿60d不会导致栉孔扇贝的大批死亡;饥饿对栉孔扇贝肥满度的影响较大,以饥饿10d后最为明显;饥饿对不同组织营养组成的影响首先表现在内脏团粗脂肪相对含量的急剧下降和蛋白质相对含量的增加;饥饿期间的O:N 比(耗氧量/排氨量)在饥饿20d后下降到最低值,然后又有所回升。实验期间,O:N比小于10,这表明在饥饿期间栉孔扇贝以蛋白质代谢为主。实验部分 II:滤食性贝类对何种来源的蛋白质摄食、吸收较佳,以及滤食性贝类对蛋白质和糖类的需求情况都是值得注意的问题。本实验配制了三种配合饲料,其中两种参照滤食性贝类天然饵料单胞藻的营养成分,其蛋白源分别为植物性原料(螺旋藻粉和豆粉)和动物性原料(鱼粉和贝边粉);另外还配制了主要成分为糖类的地瓜粉饲料。在研究不同营养条件下栉孔扇贝(Chlamys farreri)的摄食、同化和生长情况的基础上,初步确定了不同蛋白源和糖类对栉孔扇区贝生长的影响。结果表明:栉孔扇贝对单胞藻组饵料的摄食率最高,以下依次是动物蛋白组,地瓜粉组和植物蛋白组。而贝类对植物蛋白饲料组的同化率最高,以下依次是动物蛋白组,单胞藻组和地瓜粉组。在不同营养条件下,栉孔扇贝的生长明显有差异。就不同规格的栉孔扇贝而言,三角褐指藻效果最好,以下依次为:动物蛋白饲料,地瓜粉饲料和植物蛋白饲料。由此可见,在配合饲料中,营养效果为:动物性蛋白> 糖类 > 植物性蛋白。不同营养条件对栉孔扇贝的内脏部分和肉柱部分生长的影响基本相同。实验部分III:应用正交实验设计法研究了黑西哥湾扇贝(Argopecten irradians concentricus)对饲料中蛋白质、糖、脂肪及维生素C的适宜需求量,并初步筛选了最佳饲料配方。实验结果表明:在本实验条件下,就黑西哥湾扇贝性腺指数,肉柱指数,内脏团指数和肥满度而言,最佳营养成分含量相同,均为:蛋白质35%,糖类39%,脂类9%,维生素C含量对贝类生长影响不大。在三种营养物质中,蛋白质含量对性腺指数和肥满度的影响最大,而糖尖含量对肉柱指数和内脏团指数的影响最大。脂类含量对贝类生长的影响相对较小。本实验结果下最佳配方为:蛋白质35%, 糖类39%,脂类9%,与实验结果中各营养物质的最优水平一致。
其他摘要Nutriology is an important component of biology study. The thorough studies of the nutrition requirement of the animal are the assurance to the success of its culture in future. The shellfish nutriology is studied very late, and it is backward to the fish and shrimp nutriology study. This paper mainly includes three parts as follows: Part I: The shellfish nutriology studies are always processing by stressing one or more nutrition, and the physiology action of shellfish under starvation is less reported. The effects of starvation (for 60 days) on the condition index, nutrient composition of different tissues, and metabolism of the scallop Chlamys farreri were studied in laboratory from Oct. 17 to Dec. 15, 1997. The results showed that the scallops of two tanks have not mass mortality during the experiment, and survival rates of control Group and starvation Group were 93.5% and 92.0%, respectively. Effect of starvation on the meat condition of the scallops was strong, especially after 10 days. The lipid percentage of dropped sharply while the protein percentage increased. Our results show that the impact of starvation on the oxygen consumption rate (OCR) and the ammonia-N excretion rate (AER) were obvious too. The O:N ratios varied in different degrees, and dropped to the lowest value after 20 days. The ratios, which was lower than 10, suggested that the protein was the main resource for metabolism. Part II: the ingestion rate, assimilation efficiency and growth rate of scallop, Chlamys farreri, feeded with different diet were studied. Three artificial diets were confected. Two of them were consulted to the nutritional component of the nature diet of shellfish: unicellular algae, whose protein were plant source and animal source, respectively. And a pachyrhizus powder diet, which is mainly contained carbohydrate, was confected too. The results show that: the highest Ingestion Rate was occurred in the algae group, thereinafter are animal protein diet group, pachyrhizus powder diet group and plant protein diet group in turn. The highest assimilation efficiency was occurred in the plant protein diet group thereinafter are animal protein diet group, algae group and pachyrhizus powder diet group in turn. Under different diet condition, there is distinctly difference of the scallop growth. In all the three groups, the highest growth rate was occurred in the algae group, thereinafter are animal protein diet group, pachyrhizus powder diet group and plant protein diet group in turn. Thus it can be seen, in the artificial diets, the nutrition effect are animal protein > carbohydrate > plant protein. The impact of different diets to the growth of viscera and adductor muscle of scallop is the same. Part III: the nutrition requirements of southern bay scallop, Argopecten irradians concentricus, to protein, carbohydrate, lipid and vitamin C were studied, and the best diet prescription was selected. The results show that: under the experimental condition, to the condition index and the condition index of dried viscera, dried gonad, dried adductor muscle of the scallop, the best nutrition levels are the same: protein 35%, carbohydrate 39%, lipid 9%, and the vitamin C doesn't have much effect. To the condition index and the condition index of dried gonad, the protein level is the most important, and to the condition index of dried viscera and dried adductor muscle the carbohydrate level is the most important. The lipid level effects the condition indexes somewhat less. The best artificial prescription is: protein 35%, carbohydrate 39%, lipid 9%, as the same as the best nutrition level.
页数54
语种中文
文献类型学位论文
条目标识符http://ir.qdio.ac.cn/handle/337002/1549
专题海洋生物技术研发中心
推荐引用方式
GB/T 7714
王健. 扇贝营养需求与代用饲料研究[D]. 中国科学院海洋研究所. 中国科学院海洋研究所,1999.
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