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Proteomic analysis highlights the immune responses of the hepatopancreas against Hematodinium infection in Portunus trituberculatus
Li, Meng1,2; Wang, Jinfeng1,2,3; Huang, Qian1,2,3; Li, Caiwen1,2,3,4
2019-04-15
Source PublicationJOURNAL OF PROTEOMICS
ISSN1874-3919
Volume197Pages:92-105
Corresponding AuthorLi, Caiwen(cwli@qdio.ac.cn)
AbstractThe parasitic dinoflagellate Hematodinium is considered an important pathogen of economically important marine crustaceans and has been reported from many wild and cultured species. While limited studies have been conducted to reveal the host-parasite interaction in crustaceans, the underlying molecular mechanisms between Hematodinium and its crustacean hosts are scarcely known. We conducted a comprehensive study to investigate the proteomic responses to Hematodinium infection in the hepatopancreas of Portunus trituberculatus using an iTRAQ-based quantitative proteomic technology. A total of 905 identified proteins including 392 differentially expressed proteins (DEPs) were subjected to GO, COG and KEGG-pathway enrichment analysis, with sixteen DEPs further validated by quantitative real-time PCR. Hematodinium parasites resulted in immune-suppressive and adverse effects on affected hosts, thorough inhibition of the important pattern recognition receptors (C-lectin, SR class B, and Toll)-mediated immune responses, regulation of the complement and coagulation pathway, dysregulation of important cell adhesion molecules and extracellular matrix, and imbalance of the cellular redox homeostasis in the hepatopancreas of affected crabs. Moreover, the lysosomes pathway was dysregulated seriously in the hepatopancreas of P. trituberculatus post Hematodinium challenge. The results provided evidences on how the Hematodinium parasite overcame the innate immunity of P. trituberculatus and caused pathological alteration in affected tissues Biological significance: The manuscript presented the first iTRAQ-based proteomic study of the host-parasite interaction between an important marine crustacean and the parasitic dinoflagellate Hematodinium The manuscript reported the key pathways and proteins involved in the host-parasite interactions. The major findings will contribute to the better understanding of the molecular mechanism of the particular host-parasite interaction, as wells as the pathogenic process in susceptible tissues of affected crustacean hosts.
KeywordCrustacean Parasite iTRAQ labeling Proteomic profile Host-parasite interaction
DOI10.1016/j.jprot.2018.11.012
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China (NSFC)[41676102] ; CPSF-CAS Joint Foundation[2016LH0034] ; China Postdoctoral Science Foundation[2016M600561]
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000462108200010
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/154945
Collection海洋生态与环境科学重点实验室
Corresponding AuthorLi, Caiwen
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266077, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 200049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Meng,Wang, Jinfeng,Huang, Qian,et al. Proteomic analysis highlights the immune responses of the hepatopancreas against Hematodinium infection in Portunus trituberculatus[J]. JOURNAL OF PROTEOMICS,2019,197:92-105.
APA Li, Meng,Wang, Jinfeng,Huang, Qian,&Li, Caiwen.(2019).Proteomic analysis highlights the immune responses of the hepatopancreas against Hematodinium infection in Portunus trituberculatus.JOURNAL OF PROTEOMICS,197,92-105.
MLA Li, Meng,et al."Proteomic analysis highlights the immune responses of the hepatopancreas against Hematodinium infection in Portunus trituberculatus".JOURNAL OF PROTEOMICS 197(2019):92-105.
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