Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Genome-wide identification of genes involved in carbon fixation in Saccharina japonica and responses of putative C-4-related genes to bicarbonate concentration and light intensity | |
Shao, Zhanru1,2,3; Wang, Wenli4; Zhang, Pengyan1,5 ; Yao, Jianting1,2,3; Wang, Fahe4; Duan, Delin1,2,3,4
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| 2019-04-01 | |
| 发表期刊 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
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| ISSN | 0981-9428 |
| 卷号 | 137页码:75-83 |
| 通讯作者 | Duan, Delin(dlduan@qdio.ac.cn) |
| 摘要 | Brown algae play a dominant role hi the primary productivity of coastal ecosystems and may have an efficient carbon fixation. In this work, 56 genes involved in Inorganic carbon fixation were identified from the Saccharine japonica genome. Sequence structure analysis of these genes showed the existence of corresponding function domains and active amino acid sites highly conserved with other stramenopile species. The predicted subcellular localizations showed that Calvin cycle-related enzymes predominantly reside in the plastid and that putative C-4-related enzymes are mainly distributed in the mitochondrion. We determined the transcriptional profiles and enzymatic activities of these C-4-related enzymes in response to the KHCO3 concentrations and light intensities. Pyruvate orthophosphate dikinase (PPDK) presented the greatest response to low HCO3- concentrations and high light intensity. Phosphoenolpyruvate carboxykinase (PEPCK) was up-regulated at low HCO3- concentrations to compensate for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and might be the crucial decarboxylase in this kelp. We propose that S. japonica might possess a PPDK- and PEPCK-dependent C-4-like pathway that enables its rapid growth in natural coastal environments. |
| 关键词 | Carbon fixation Environmental influences Enzymatic activity mRNA abundance Saccharina japonica |
| DOI | 10.1016/j.plaphy.2019.01.032 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | CAS-Fujian STS Project[2017T3012] ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Qingdao National Laboratory for Marine Science and Technology Project[2018ASKJ03] ; Shandong Key Research and Development Program[2018GHY115023] ; Shandong Key Research and Development Program[2018GHY115023] ; Qingdao National Laboratory for Marine Science and Technology Project[2018ASKJ03] ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; CAS-Fujian STS Project[2017T3012] |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| WOS记录号 | WOS:000462106100007 |
| 出版者 | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/154941 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Duan, Delin |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Waded Roa, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Qingdao Brightmoon Seaweed Grow Co Ltd, State Key Lab Bioact Seaweed Subst, 777 Mingyue Rd, Qingdao 266400, Peoples R China 5.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, 106 Nanjing Rood, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Shao, Zhanru,Wang, Wenli,Zhang, Pengyan,et al. Genome-wide identification of genes involved in carbon fixation in Saccharina japonica and responses of putative C-4-related genes to bicarbonate concentration and light intensity[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2019,137:75-83. |
| APA | Shao, Zhanru,Wang, Wenli,Zhang, Pengyan,Yao, Jianting,Wang, Fahe,&Duan, Delin.(2019).Genome-wide identification of genes involved in carbon fixation in Saccharina japonica and responses of putative C-4-related genes to bicarbonate concentration and light intensity.PLANT PHYSIOLOGY AND BIOCHEMISTRY,137,75-83. |
| MLA | Shao, Zhanru,et al."Genome-wide identification of genes involved in carbon fixation in Saccharina japonica and responses of putative C-4-related genes to bicarbonate concentration and light intensity".PLANT PHYSIOLOGY AND BIOCHEMISTRY 137(2019):75-83. |
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