Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Transcriptome analysis reveals the activation of neuroendocrine-immune system in shrimp hemocytes at the early stage of WSSV infection | |
Wang, Fuxuan1,2,3; Li, Shihao1,2,4; Xiang, Jianhai1,2 ; Li, Fuhua1,2,4
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| 2019-03-28 | |
| 发表期刊 | BMC GENOMICS
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| ISSN | 1471-2164 |
| 卷号 | 20页码:14 |
| 通讯作者 | Li, Fuhua(fhli@qdio.ac.cn) |
| 摘要 | Background: Functional communications between nervous, endocrine and immune systems are well established in both vertebrates and invertebrates. Circulating hemocytes act as fundamental players in this crosstalk, whose functions are conserved during the evolution of the main groups of metazoans. However, the roles of the neuroendocrineimmune (NEI) system in shrimp hemocytes during pathogen infection remain largely unknown. Results: In this study, we sequenced six cDNA libraries prepared with hemocytes from Litopenaeus vannamei which were injected by WSSV (white spot syndrome virus) or PBS for 6 h using Illumina Hiseq 4000 platform. As a result, 3444 differentially expressed genes (DEGs), including 3240 up-regulated genes and 204 down-regulated genes, were identified from hemocytes after WSSV infection. Among these genes, 349 DEGs were correlated with innate immunity and categorized into seven groups based on their predictive function. Interestingly, 18 genes encoded putative neuropeptide precursors were induced significantly by WSSV infection. Furthermore, some genes were mapped to several typical processes in the NEI system, including proteolytic processing of prohormones, amino acid neurotransmitter pathways, biogenic amine biosynthesis and acetylcholine signaling pathway. Conclusions: The data suggested that WSSV infection triggers the activation of NEI in shrimp, which throws a light on the pivotal roles of NEI system mediated by hemocytes in shrimp antiviral immunity. |
| 关键词 | Litopenaeus vannamei Hemocytes WSSV Neuroendocrine-immune system |
| DOI | 10.1186/s12864-019-5614-4 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | China Agriculture Research system-48 (CARS-48) ; National Natural Science Foundation of China[41776158] ; National Natural Science Foundation of China[31772880] ; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science and Technology[MS2017NO04] ; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science and Technology[MS2017NO04] ; National Natural Science Foundation of China[31772880] ; National Natural Science Foundation of China[41776158] ; China Agriculture Research system-48 (CARS-48) |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS类目 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS记录号 | WOS:000463242500003 |
| 出版者 | BMC |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/154938 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Li, Fuhua |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Fuxuan,Li, Shihao,Xiang, Jianhai,et al. Transcriptome analysis reveals the activation of neuroendocrine-immune system in shrimp hemocytes at the early stage of WSSV infection[J]. BMC GENOMICS,2019,20:14. |
| APA | Wang, Fuxuan,Li, Shihao,Xiang, Jianhai,&Li, Fuhua.(2019).Transcriptome analysis reveals the activation of neuroendocrine-immune system in shrimp hemocytes at the early stage of WSSV infection.BMC GENOMICS,20,14. |
| MLA | Wang, Fuxuan,et al."Transcriptome analysis reveals the activation of neuroendocrine-immune system in shrimp hemocytes at the early stage of WSSV infection".BMC GENOMICS 20(2019):14. |
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| Transcriptome analys（1588KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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