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Tissue-specific molecular and cellular toxicity of Pb in the oyster (Crassostrea gigas): mRNA expression and physiological studies
Meng, Jie1,2,4; Wang, Wen-Xiong5; Li, Li1,2,4; Zhang, Guofan1,3,4
2018-05-01
发表期刊AQUATIC TOXICOLOGY
ISSN0166-445X
卷号198期号:2018页码:257-268
通讯作者Li, Li ; Zhang, Guofan
摘要

Lead (Pb) is one of the ubiquitous and toxic elements in aquatic environment. In oysters, gills and digestive glands are the main target organs for Pb-induced toxicity, but there is limited information on the molecular mechanisms underlying its toxicity. The present study investigated the Pb-induced toxicity mechanisms in the Pacific oyster (Crassostrea gigas) based on transcriptome, phenotypic anchoring, and validation of targeted gene expression. Gene ontology and pathway enrichment analyses revealed the differential Pb toxicity mechanisms in the tissues. In the gills, Pb disturbed the protein metabolism, with the most significant enrichment of the "protein processing in endoplasmic reticulum" pathway. The main mechanism comprised of a Pb-stimulated calcium (Ca2+) increase by the up-regulation of transporter-Ca-ATPase expression. The disturbed Ca2+ homeostasis then further induced high expressions of endoplasmic reticulum (ER) chaperones, leading to ER stress in the oysters. Unfolded proteins induced ER associated degradation (ERAD), thereby preventing the accumulation of folding incompetent glycoproteins. However, Pb mainly induced oxidative reduction reactions in the digestive gland with high accumulation of lipid peroxidation products and high expression of antioxidant enzymes. Further, Pb induced fatty acid beta-oxidation and CYP450 catalyzed co-oxidation due to increased metabolic expenditure for detoxification. The increased content of arachidonic acid indicated that Pb exposure might alter unsaturated fatty acid composition and disturb cellular membrane functions. Taken together, our results provided a new insight into the molecular mechanisms underlying Pb toxicity in oysters.

关键词Crassostrea Pb Exposure Er Stress Fatty Acid Oxidation Transcriptome
DOI10.1016/j.aquatox.2018.03.010
语种英语
资助项目Shandong Provincial Natural Science Foundation, China[ZR2016DQ13] ; National Natural Science Foundation of China[31530079] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-48] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03] ; Strategic Priority Research Program of
WOS研究方向Marine & Freshwater Biology ; Toxicology
WOS类目Marine & Freshwater Biology ; Toxicology
WOS记录号WOS:000430630100026
出版者ELSEVIER SCIENCE BV
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/154489
专题实验海洋生物学重点实验室
通讯作者Li, Li; Zhang, Guofan
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao, Shandong, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
4.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China
5.HKUST Shenzhen Res Inst, Marine Environm Lab, Shenzhen 518057, Peoples R China
推荐引用方式
GB/T 7714
Meng, Jie,Wang, Wen-Xiong,Li, Li,et al. Tissue-specific molecular and cellular toxicity of Pb in the oyster (Crassostrea gigas): mRNA expression and physiological studies[J]. AQUATIC TOXICOLOGY,2018,198(2018):257-268.
APA Meng, Jie,Wang, Wen-Xiong,Li, Li,&Zhang, Guofan.(2018).Tissue-specific molecular and cellular toxicity of Pb in the oyster (Crassostrea gigas): mRNA expression and physiological studies.AQUATIC TOXICOLOGY,198(2018),257-268.
MLA Meng, Jie,et al."Tissue-specific molecular and cellular toxicity of Pb in the oyster (Crassostrea gigas): mRNA expression and physiological studies".AQUATIC TOXICOLOGY 198.2018(2018):257-268.
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