IOCAS-IR  > 实验海洋生物学重点实验室
Tissue-specific molecular and cellular toxicity of Pb in the oyster (Crassostrea gigas): mRNA expression and physiological studies
Meng, Jie1,2,4; Wang, Wen-Xiong5; Li, Li1,2,4; Zhang, Guofan1,3,4
2018-05-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume198Issue:2018Pages:257-268
Corresponding AuthorLi, Li ; Zhang, Guofan
Abstract

Lead (Pb) is one of the ubiquitous and toxic elements in aquatic environment. In oysters, gills and digestive glands are the main target organs for Pb-induced toxicity, but there is limited information on the molecular mechanisms underlying its toxicity. The present study investigated the Pb-induced toxicity mechanisms in the Pacific oyster (Crassostrea gigas) based on transcriptome, phenotypic anchoring, and validation of targeted gene expression. Gene ontology and pathway enrichment analyses revealed the differential Pb toxicity mechanisms in the tissues. In the gills, Pb disturbed the protein metabolism, with the most significant enrichment of the "protein processing in endoplasmic reticulum" pathway. The main mechanism comprised of a Pb-stimulated calcium (Ca2+) increase by the up-regulation of transporter-Ca-ATPase expression. The disturbed Ca2+ homeostasis then further induced high expressions of endoplasmic reticulum (ER) chaperones, leading to ER stress in the oysters. Unfolded proteins induced ER associated degradation (ERAD), thereby preventing the accumulation of folding incompetent glycoproteins. However, Pb mainly induced oxidative reduction reactions in the digestive gland with high accumulation of lipid peroxidation products and high expression of antioxidant enzymes. Further, Pb induced fatty acid beta-oxidation and CYP450 catalyzed co-oxidation due to increased metabolic expenditure for detoxification. The increased content of arachidonic acid indicated that Pb exposure might alter unsaturated fatty acid composition and disturb cellular membrane functions. Taken together, our results provided a new insight into the molecular mechanisms underlying Pb toxicity in oysters.

KeywordCrassostrea Pb Exposure Er Stress Fatty Acid Oxidation Transcriptome
DOI10.1016/j.aquatox.2018.03.010
Language英语
Funding ProjectShandong Provincial Natural Science Foundation, China[ZR2016DQ13] ; National Natural Science Foundation of China[31530079] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-48] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03] ; Strategic Priority Research Program of
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000430630100026
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/154489
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Li; Zhang, Guofan
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao, Shandong, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
4.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China
5.HKUST Shenzhen Res Inst, Marine Environm Lab, Shenzhen 518057, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Meng, Jie,Wang, Wen-Xiong,Li, Li,et al. Tissue-specific molecular and cellular toxicity of Pb in the oyster (Crassostrea gigas): mRNA expression and physiological studies[J]. AQUATIC TOXICOLOGY,2018,198(2018):257-268.
APA Meng, Jie,Wang, Wen-Xiong,Li, Li,&Zhang, Guofan.(2018).Tissue-specific molecular and cellular toxicity of Pb in the oyster (Crassostrea gigas): mRNA expression and physiological studies.AQUATIC TOXICOLOGY,198(2018),257-268.
MLA Meng, Jie,et al."Tissue-specific molecular and cellular toxicity of Pb in the oyster (Crassostrea gigas): mRNA expression and physiological studies".AQUATIC TOXICOLOGY 198.2018(2018):257-268.
Files in This Item:
File Name/Size DocType Version Access License
Tissue-specific mole(1577KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Meng, Jie]'s Articles
[Wang, Wen-Xiong]'s Articles
[Li, Li]'s Articles
Baidu academic
Similar articles in Baidu academic
[Meng, Jie]'s Articles
[Wang, Wen-Xiong]'s Articles
[Li, Li]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Meng, Jie]'s Articles
[Wang, Wen-Xiong]'s Articles
[Li, Li]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Tissue-specific molecular and cellular toxicity of Pb in the oyster (Crassostrea gigas)_ mRNA expression and physiological studies.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.