Thesis Advisor王斌贵
Degree Grantor中国科学院大学
Place of Conferral中国科学院海洋研究所
Degree Name工程硕士
Keyword地中海 溜曲霉 焦曲霉 神经氨酸酶活性


本论文对分离自地中海海鞘(Pyura momus)的一株焦曲霉(Aspergillus ustusTK-5进行了规模发酵,从中分离鉴定了27个化合物,包括8个混源萜类化合物(AU 18)、10个倍半萜类衍生物(AU 918)、7个二倍半萜类化合物(AU 1925)、1个三萜类化合物(AU 26)和1个二酮哌嗪类化合物(AU 27)。其中三个混源萜类化合物(AU 13)经核磁共振、单晶衍射实验确定为新化合物,并对其生源途径进行了推测。另外对地中海海绵(Ircina oros)来源的一株溜曲霉(Aspergillus tamariiTK-18进行了研究,从其发酵产物中分离鉴定了6个化合物(AL 16),包括甾醇和二酮哌嗪类化合物。分别对上述萜类化合物进行了抗菌活性和神经氨酸酶抑制活性、乙酰胆碱酯酶抑制活性测试,结果表明混源萜类化合物对神经氨酸酶的抑制活性较强,其中化合物AU 1AU 2AU 4AU 6AU 7IC50值分别为5.288.955.711.378.95 μM,化合物AU 4对小麦赤霉病菌(Fusarium graminearum)具有较强的抑制活性(MIC = 8.0 μg/mL)。


Other Abstract

Because of unique high salt and oligotrophic environment, Mediterranean Sea become one of the main regions for natural products research. Recently, researches on the secondary metabolites of marine animal-derived microorganisms continuously increased. This thesis systematically investigates and analyzes the natural products of marine animal-derived fungi from the Mediterranean Sea since 2000. Statistical analysis found that the main types of those natural products are polyketones and terpenes, respectively accounting for 49% and 25%, the total amount of other types of compounds is about 26%, which generally exhibited antibacterial, antitumor, and enzyme inhibitiory activities. Furthermore, the proportion of polyketides with biological activity has rised to 53%. It has been proved that the Mediterranean Sea is a huge treasure resource of active natural products and has great research potential.

Cultivation of the fungal strain Aspergillus ustus TK-5, which was isolated from the fresh tissue of Mediterranean ascidian Pyura momus, resulted in the identification of 27 compounds, including eight meroterpenoids (AU 18), ten sesquiterpenoids (AU 918), seven sesquiterpenes (AU 1925), one triterpenoid (AU 26), and one diketopiperazine (AU 27). The meroterpenoids (AU 13) were identified as new compounds by NMR and single crystal diffraction, and their biogenic pathways were speculated. The secondary metabolites of Aspergillus tamarii TK-18 isolated from Mediterranean sponge Ircina oros were studied. Six compounds (AL 1-6) were isolated and identified from their fermentation products, including sterols and diketopiperazines. All terpenes were tested for neuraminidase inhibitory and acetylcholinesterase inhibitory activity. AU 1, AU 2, AU 4, AU 6, and AU 7 were found to be highly active for neuraminidase, with IC50 values of 5.28, 8.95, 5.71, 1.37, 8.95 μM. The antibacterial activity of meroterpenoids was evaluated and it was found that only AU 4MIC=8.0 μg/mL)had significant antibacterial activity against Fusarium graminearum.

In summary, the identification and biological activities of compounds from two fungi which from Turkish sea ascidian and sponge further confirmed the diversity of the chemical structures and biological activities of metabolites from Mediterranean-derived fungi. It enriches the research content of marine natural products and provides a certain theoretical and experimental basis for the development and utilization of marine biological resources.

MOST Discipline Catalogue工学 ; 工学::生物工程
Document Type学位论文
Recommended Citation
GB/T 7714
李莉. 两株地中海海鞘和海绵来源的内生真菌次级代谢产物及其生物活性研究[D]. 中国科学院海洋研究所. 中国科学院大学,2018.
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