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题名: Heterogeneity of intron presence/absence in Olifantiella sp (Bacillariophyta) contributes to the understanding of intron loss
作者: Han, Jichang1; Zhang, Lin2; Wang, Pu3; Yang, Guanpin4; Wang, Song1; Li, Yuhang5; Pan, Kehou1,6
刊名: JOURNAL OF PHYCOLOGY
出版日期: 2018-02-01
卷号: 54, 期号:1, 页码:105-113
关键词: 18S rRNA ; diatom ; intron evolution ; intron loss ; spliceosomal intron
DOI: 10.1111/jpy.12605
文章类型: Article
英文摘要: Although hypotheses have been proposed and developed to interpret the origins and functions of introns, substantial controversies remain about the mechanism of intron evolution. The availability of introns in the intermediate state is quite helpful for resolving this debate. In this study, a new strain of diatom (denominated as DB21-1) was isolated and identified as Olifantiella sp., which possesses multiple types of 18S rDNAs (obtained from genomic DNA; lengths ranged from 2,056bp to 2,988bp). Based on alignments between 18S rDNAs and 18S rRNA (obtained from cDNA; 1,783bp), seven intron insertion sites (IISs) located in the 18S rDNA were identified, each of which displayed the polymorphism of intron presence/absence. Specific primers around each IIS were designed to amplify the introns and the results indicated that introns in the same IIS varied in lengths, while terminal sequences were conserved. Our study showed that the process of intron loss happens via a series of successive steps, and each step could derive corresponding introns under intermediate states. Moreover, these results indicate that the mechanism of genomic deletion that occurs at DNA level can also lead to exact intron loss.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000424094000011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.qdio.ac.cn/handle/337002/154358
Appears in Collections:海洋生物分类与系统演化实验室_期刊论文

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作者单位: 1.Ocean Univ China, Lab Appl Microalgae Biol, Qingdao 266003, Peoples R China
2.Ningbo Univ, Coll Marine, Ningbo 315211, Zhejiang, Peoples R China
3.Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA
4.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
5.Chinese Acad Sci, Dept Marine Organism Taxon & Phylogeny, Inst Oceanol, Qingdao 266071, Peoples R China
6.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266003, Peoples R China
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