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Histones and chymotrypsin-like elastases play significant roles in the antimicrobial activity of tongue sole neutrophil extracellular traps
Wen, Li-lian1,2,3; Zhao, Ming-li1,2,3; Chi, Heng1,2; Sun, Li1,2
2018
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号72页码:470-476
文章类型Article
摘要Neutrophil extracellular traps (NETs) are a form of extracellular antimicrobial structure of neutrophils observed in higher and lower vertebrates, the latter including the teleost fish tongue sole Cynoglossus semilaevis. However, the antimicrobial mechanism of fish NETs is unknown. In the present study, we examined the potential contribution of histones and elastases to the antibacterial effect of tongue sole NETs. For this purpose, two histones (CsH2B and CsH4) and two elastases (CsElal and CsEla2) of tongue sole were investigated. The histones and elastases possess the conserved domain structures characteristic of that of histones H2B/H4 and trypsin-like serine protease, respectively. Recombinant CsH2B, CsH4, CsElal, and CsEla2 bound a wide range of Gram-negative and Gram-positive bacteria, and some of the bound bacteria were inhibited in growth by the bound histones/elastases. CsH2B, CsH4, CsElal, and CsEla2 were all localized in NETs induced by various stimuli including bacterial pathogen. Treatment of NETs with antibodies targeting CsH2B, CsH4, CsElal, and CsEla2 significantly reduced the antimicrobial effect of NETs. These results indicate that histones and chymotrypsin-like elastases are fundamental components of teleost NETs that play important roles in the antimicrobial activity of NETs.
关键词Neutrophil Extracellular Traps Histone Elastase Cynoglossus Semilaevis Antimicrobial
DOI10.1016/j.fsi.2017.11.004
收录类别SCI
语种英语
WOS记录号WOS:000423644600055
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文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/154339
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
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Wen, Li-lian,Zhao, Ming-li,Chi, Heng,et al. Histones and chymotrypsin-like elastases play significant roles in the antimicrobial activity of tongue sole neutrophil extracellular traps[J]. FISH & SHELLFISH IMMUNOLOGY,2018,72:470-476.
APA Wen, Li-lian,Zhao, Ming-li,Chi, Heng,&Sun, Li.(2018).Histones and chymotrypsin-like elastases play significant roles in the antimicrobial activity of tongue sole neutrophil extracellular traps.FISH & SHELLFISH IMMUNOLOGY,72,470-476.
MLA Wen, Li-lian,et al."Histones and chymotrypsin-like elastases play significant roles in the antimicrobial activity of tongue sole neutrophil extracellular traps".FISH & SHELLFISH IMMUNOLOGY 72(2018):470-476.
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