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The dUTPase of white spot syndrome virus assembles its active sites in a noncanonical manner
Zang, Kun1,2,3; Li, Fuhua1,2; Ma, Qingjun1,2
2018-01-19
发表期刊JOURNAL OF BIOLOGICAL CHEMISTRY
卷号293期号:3页码:1088-1099
文章类型Article
摘要dUTPases are essential enzymes for maintaining genome integrity and have recently been shown to play moonlighting roles when containing extra sequences. Interestingly, the trimeric dUTPase of white spot syndrome virus (wDUT) harbors a sequence insert at the position preceding the C-terminal catalytic motifV(pre-V insert), rarely seen in other dUTPases. However, whether this extra sequence endowswDUTwith additional properties is unknown. Herein, we present the crystal structures of wDUT in both ligand-free and ligand-bound forms. We observed that the pre-V insert in wDUT forms an unusual beta-hairpin structure in the domain-swapping region and thereby facilitates a unique orientation of the adjacent C-terminal segment, positioning the catalytic motif V onto the active site of its own subunit instead of a third subunit. Consequently, wDUT employs two-subunit active sites, unlike the widely accepted paradigm that the active site of trimeric dUTPase is contributed by all three subunits. According to results from local structural comparisons, the active-site configuration ofwDUTis similar to that of known dUTPases. However, we also found that residues in the second-shell region of the active site are reconfigured in wDUT as an adaption to its unique C-terminal orientation. We also show that deletion of the pre-V insert significantly reduces wDUT's enzymatic activity and thermal stability. We hypothesize that this rare structural arrangement confers additional functionality to wDUT. In conclusion, our study expands the structural diversity in the conserved dUTPase family and illustrates how sequence insertion and amino acid substitution drive protein evolution cooperatively.
DOI10.1074/jbc.M117.815266
收录类别SCI
语种英语
WOS记录号WOS:000422864500027
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/154331
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Nanhai Rd 7, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Zang, Kun,Li, Fuhua,Ma, Qingjun. The dUTPase of white spot syndrome virus assembles its active sites in a noncanonical manner[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2018,293(3):1088-1099.
APA Zang, Kun,Li, Fuhua,&Ma, Qingjun.(2018).The dUTPase of white spot syndrome virus assembles its active sites in a noncanonical manner.JOURNAL OF BIOLOGICAL CHEMISTRY,293(3),1088-1099.
MLA Zang, Kun,et al."The dUTPase of white spot syndrome virus assembles its active sites in a noncanonical manner".JOURNAL OF BIOLOGICAL CHEMISTRY 293.3(2018):1088-1099.
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