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题名: Two novel LRR and Ig domain-containing proteins from oyster Crassostrea gigas function as pattern recognition receptors and induce expression of cytokines
作者: Wang XiuDan(王秀丹)1,4; Mengqiang Wang(王孟强)1; Xu, Qingsong3; Xu JiaChao(徐家超)1,4; Lv, Zhao1,4; Wang, Lingling3; Song, Linsheng2,3
刊名: FISH & SHELLFISH IMMUNOLOGY
出版日期: 2017-11-01
卷号: 70, 页码:308-318
关键词: Crassostrea gigas ; Leucine-rich repeat domain ; Immunoglobulin domain ; Pattern recognition receptor ; Innate immunity
DOI: 10.1016/j.fsi.2017.09.023
文章类型: Article
英文摘要: Leucine-rich repeat (LRR) domain and immunoglobulin (Ig) domain are both competent immune recognition modules, and the immunological roles of LRR and Ig domain containing-proteins (LRRIGs) are speculated to be multifunctional and worth investigating. In the present study, two novel LRRIGs, CgLRRIG-1 and CgLRRIG-2, were identified and characterized from oyster Crassostrea gigas. Both of them contained an N-terminal LRR region, an Ig domain, a transmembrane region, and a C-terminal cytoplasmic tail. The mRNA transcripts of CgLRRIG-1 and CgLRRIG-2 were constitutively expressed in muscle, gill, hepatopancreas, mantle, gonad and hemocytes with the highest expression level in hepatopancreas. Their mRNA expression levels in hemocytes were significantly up-regulated after the stimulations with four PAMPs including peptidoglycan (PGN), lipopolysaccharide (LPS), glucan (GLU) and polyinosinic-polycytidylic acid (poly I:C) and one bacteria Vibrio anguillarum. The recombinant proteins, rCgLRRIG-1 and rCgLRRIG-2, could bind to PGN, LPS, GLU and poly I:C, and rCgLRRIG-2 exhibited higher binding affinity. Additionally, rCgLRRIG-1 and rCgLRRIG-2 could significantly induce the expression of CgTNF-1 and CgIL17-5 in cultured oyster hemocytes, and the activity of rCgLRRIG-2 was higher than that of rCgLRRIG-1. All these results indicated that CgLRRIG-1 and CgLRRIG-2 could function as immune effectors or pro-inflammatory factors as well as PRRs in oyster. (C) 2017 Elsevier Ltd. All rights reserved.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000416186800032
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.qdio.ac.cn/handle/337002/143293
Appears in Collections:实验海洋生物学重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China
3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Wang, Xiudan,Wang, Mengqiang,Xu, Qingsong,et al. Two novel LRR and Ig domain-containing proteins from oyster Crassostrea gigas function as pattern recognition receptors and induce expression of cytokines[J]. FISH & SHELLFISH IMMUNOLOGY,2017,70:308-318.
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文件名: Two novel LRR and Ig domain-containing proteins from oyster Crassostrea gigas function as pattern recognition receptors and induce expression of cytokines.pdf
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