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Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining
Chi, Heng1,4; Wen, Li-lian1,3; Sui, Zhi-Hai1,3; Sun, Qing-Lei1,2; Sun, Li1,2,4
2017-12-01
Source PublicationTISSUE & CELL
Volume49Issue:6Pages:751-755
SubtypeArticle
AbstractCytomorphological and cytochemical staining are important methods for the identification of cell types, in particular in fish which often lack biological tools such as specific antibodies. Myeloperoxidase (MPO) is usually used as an intracellular marker of neutrophil accumulation in tissues and a marker of neutrophil activity in plasma. In this study, we reported a potassium iodide and oxidized pyronine Y (KI-PyY) staining method for rapid and highly sensitive detection of MPO-positive cells in turbot blood, peritoneum, and tissues. MPO-positive cells, which mostly represented neutrophils, were stained brown and clearly distinguished from other cells, such as lymphocytes, monocytes, and macrophages, which were stained pink. Following bacterial stimulation, the proportions of neutrophils were 27.49% and 38.05% in peripheral blood leukocytes and peritoneum, respectively, judging by the stained MPO. Kidney granulocytes contained abundant MPO-positive cells which were probably immature neutrophils with low expression of MPO. It is noteworthy that MPO-positive cells were detected in the tissue sections of kidney, spleen, and gut, with distribution profiles specific to each tissue. However, the cell morphology was not distinct in the stained tissue sections. These results indicate that the KI-PyY staining method is highly sensitive, applicable to different types of samples, and will be useful for the study of neutrophils in different compartments of fish.
KeywordCytochemical Identification Ki-pyy Turbot Mpo-positive Granulocytes
DOI10.1016/j.tice.2017.10.008
Indexed BySCI
Language英语
WOS IDWOS:000417042700015
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143292
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
First Author Affilication中国科学院海洋研究所
Recommended Citation
GB/T 7714
Chi, Heng,Wen, Li-lian,Sui, Zhi-Hai,et al. Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining[J]. TISSUE & CELL,2017,49(6):751-755.
APA Chi, Heng,Wen, Li-lian,Sui, Zhi-Hai,Sun, Qing-Lei,&Sun, Li.(2017).Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining.TISSUE & CELL,49(6),751-755.
MLA Chi, Heng,et al."Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining".TISSUE & CELL 49.6(2017):751-755.
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