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Transcriptomic and morphological analyses of Litopenaeus vannamei intestinal barrier in response to Vibrio paraheamolyticus infection reveals immune response signatures and structural disruption
Qi, Cancan1,2,3; Wang, Lei1,3; Liu, Mei1,3; Jiang, Keyong1,3; Wang, Mengqiang1,3; Zhao, Wei1,3; Wang, Baojie1,3
2017-11-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号70页码:437-450
文章类型Article
摘要The white shrimp Litopenaeus vannamei has been greatly impacted by Vibrio infection. In this study, we investigated the intestinal barrier response of L vannamei following challenge with Vibrio parahaemolyticus El, by examining morphological changes and transcriptome expression levels. A total of 16,4420 unigenes were obtained from RNAseq data after quality control and assembly, and 4646 differentially expressed genes (DEGs) were identified following Vibrio challenge, of which 2469 unigenes were significantly up-regulated and 2177 were significantly down-regulated. DEGs were determined to be involved in various physical, chemical and immunological intestinal barrier functions, including peritrophin, cytoskeleton and cell junction, pattern recognition receptors, antimicrobial peptide and immune signaling pathways, serine protease/protease inhibitor and prophenoloxidase system, apoptosis and phagocytosis, and antioxidant systems. Fifteen DEGs were randomly selected for validation by real-time quantitative PCR (RT-qPCR) and showed results consistent with the RNA-seq data. Intestinal epithelial cell morphology was also affected by Vibrio challenge, showing epithelial detachment, nuclear pyknosis, and destruction of cell junctions. These results improve our current understanding of the intestinal barrier function in the shrimp response to bacterial infection. (C) 2017 Elsevier Ltd. All rights reserved.
关键词Litopenaeus Vannamei Intestinal Barrier Vibrio Parahaemolyticus Transcriptome Morphology
DOI10.1016/j.fsi.2017.09.004
收录类别SCI
语种英语
WOS记录号WOS:000416186800046
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/143286
专题海洋生物技术研发中心
实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
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Qi, Cancan,Wang, Lei,Liu, Mei,et al. Transcriptomic and morphological analyses of Litopenaeus vannamei intestinal barrier in response to Vibrio paraheamolyticus infection reveals immune response signatures and structural disruption[J]. FISH & SHELLFISH IMMUNOLOGY,2017,70:437-450.
APA Qi, Cancan.,Wang, Lei.,Liu, Mei.,Jiang, Keyong.,Wang, Mengqiang.,...&Wang, Baojie.(2017).Transcriptomic and morphological analyses of Litopenaeus vannamei intestinal barrier in response to Vibrio paraheamolyticus infection reveals immune response signatures and structural disruption.FISH & SHELLFISH IMMUNOLOGY,70,437-450.
MLA Qi, Cancan,et al."Transcriptomic and morphological analyses of Litopenaeus vannamei intestinal barrier in response to Vibrio paraheamolyticus infection reveals immune response signatures and structural disruption".FISH & SHELLFISH IMMUNOLOGY 70(2017):437-450.
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