IOCAS-IR  > 海洋生物技术研发中心
Transcriptomic and morphological analyses of Litopenaeus vannamei intestinal barrier in response to Vibrio paraheamolyticus infection reveals immune response signatures and structural disruption
Qi, Cancan1,2,3; Wang, Lei1,3; Liu, Mei1,3; Jiang, Keyong1,3; Wang, Mengqiang1,3; Zhao, Wei1,3; Wang, Baojie1,3
2017-11-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume70Pages:437-450
SubtypeArticle
AbstractThe white shrimp Litopenaeus vannamei has been greatly impacted by Vibrio infection. In this study, we investigated the intestinal barrier response of L vannamei following challenge with Vibrio parahaemolyticus El, by examining morphological changes and transcriptome expression levels. A total of 16,4420 unigenes were obtained from RNAseq data after quality control and assembly, and 4646 differentially expressed genes (DEGs) were identified following Vibrio challenge, of which 2469 unigenes were significantly up-regulated and 2177 were significantly down-regulated. DEGs were determined to be involved in various physical, chemical and immunological intestinal barrier functions, including peritrophin, cytoskeleton and cell junction, pattern recognition receptors, antimicrobial peptide and immune signaling pathways, serine protease/protease inhibitor and prophenoloxidase system, apoptosis and phagocytosis, and antioxidant systems. Fifteen DEGs were randomly selected for validation by real-time quantitative PCR (RT-qPCR) and showed results consistent with the RNA-seq data. Intestinal epithelial cell morphology was also affected by Vibrio challenge, showing epithelial detachment, nuclear pyknosis, and destruction of cell junctions. These results improve our current understanding of the intestinal barrier function in the shrimp response to bacterial infection. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordLitopenaeus Vannamei Intestinal Barrier Vibrio Parahaemolyticus Transcriptome Morphology
DOI10.1016/j.fsi.2017.09.004
Indexed BySCI
Language英语
WOS IDWOS:000416186800046
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143286
Collection海洋生物技术研发中心
实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Qi, Cancan,Wang, Lei,Liu, Mei,et al. Transcriptomic and morphological analyses of Litopenaeus vannamei intestinal barrier in response to Vibrio paraheamolyticus infection reveals immune response signatures and structural disruption[J]. FISH & SHELLFISH IMMUNOLOGY,2017,70:437-450.
APA Qi, Cancan.,Wang, Lei.,Liu, Mei.,Jiang, Keyong.,Wang, Mengqiang.,...&Wang, Baojie.(2017).Transcriptomic and morphological analyses of Litopenaeus vannamei intestinal barrier in response to Vibrio paraheamolyticus infection reveals immune response signatures and structural disruption.FISH & SHELLFISH IMMUNOLOGY,70,437-450.
MLA Qi, Cancan,et al."Transcriptomic and morphological analyses of Litopenaeus vannamei intestinal barrier in response to Vibrio paraheamolyticus infection reveals immune response signatures and structural disruption".FISH & SHELLFISH IMMUNOLOGY 70(2017):437-450.
Files in This Item:
File Name/Size DocType Version Access License
Transcriptomic and m(3448KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Qi, Cancan]'s Articles
[Wang, Lei]'s Articles
[Liu, Mei]'s Articles
Baidu academic
Similar articles in Baidu academic
[Qi, Cancan]'s Articles
[Wang, Lei]'s Articles
[Liu, Mei]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Qi, Cancan]'s Articles
[Wang, Lei]'s Articles
[Liu, Mei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Transcriptomic and morphological analyses of Litopenaeus vannamei intestinal barrier in response to Vibrio paraheamolyticus infection reveals immune response signatures and structural disruption.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.