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Structure-based function prediction of the expanding mollusk tyrosinase family
Huang Ronglian1,2,4; Li Li1,3; Zhang Guofan1,3
2017-11-01
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号35期号:6页码:1454-1464
文章类型Article
摘要Tyrosinase (Ty) is a common enzyme found in many different animal groups. In our previous study, genome sequencing revealed that the Ty family is expanded in the Pacific oyster (Crassostrea gigas). Here, we examine the larger number of Ty family members in the Pacific oyster by high-level structure prediction to obtain more information about their function and evolution, especially the unknown role in biomineralization. We verified 12 Ty gene sequences from Crassostrea gigas genome and Pinctada fucata martensii transcriptome. By using phylogenetic analysis of these Tys with functionally known Tys from other molluscan species, eight subgroups were identified (CgTy_s1, CgTy_s2, MolTy_s1, MolTy-s2, MolTy-s3, PinTy-s1, PinTy-s2 and PviTy). Structural data and surface pockets of the dinuclear copper center in the eight subgroups of molluscan Ty were obtained using the latest versions of prediction online servers. Structural comparison with other Ty proteins from the protein databank revealed functionally important residues (H-A1, H-A2, H-A3, H-B1, H-B2, H-B3, Z1-Z9) and their location within these protein structures. The structural and chemical features of these pockets which may related to the substrate binding showed considerable variability among mollusks, which undoubtedly defines Ty substrate binding. Finally, we discuss the potential driving forces of Ty family evolution in mollusks. Based on these observations, we conclude that the Ty family has rapidly evolved as a consequence of substrate adaptation in mollusks.
关键词Tyrosinase Mollusk Ligand Binding Pocket Substrate Diversity Evolution
DOI10.1007/s00343-017-6066-9
收录类别SCI
语种英语
WOS记录号WOS:000415810900016
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文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/143285
专题海洋生物技术研发中心
实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266235, Peoples R China
4.GuangDong Ocean Univ, Marine Pearl Culture Lab, Fishery Coll, Zhanjiang 524088, Peoples R China
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Huang Ronglian,Li Li,Zhang Guofan. Structure-based function prediction of the expanding mollusk tyrosinase family[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2017,35(6):1454-1464.
APA Huang Ronglian,Li Li,&Zhang Guofan.(2017).Structure-based function prediction of the expanding mollusk tyrosinase family.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,35(6),1454-1464.
MLA Huang Ronglian,et al."Structure-based function prediction of the expanding mollusk tyrosinase family".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 35.6(2017):1454-1464.
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