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Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage
Xie Shijun1,2; Li Fuhua1,3; Zhang Xiaojun1; Zhang Jiquan1; Xiang Jianhai1,3
2017-11-01
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号35期号:6页码:1524-1530
文章类型Article
摘要The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus (WSSV) in shrimp. In this study, the nucleotide sequence encoding peritrophin-like protein of Litopenaeus vannamei (LvPT) was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of LvPT was predicted with the GenScript OptimumAntigen (TM) design tool. An anti-LvPT polyclonal antibody was produced and shown to specifically bind a band at similar to 27 kDa, identified as LvPT. The LvPT protein was expressed and its concentration determined. LvPT dsRNA (4 mu g per shrimp) was used to inhibit LvPT expression in shrimp, and a WSSV challenge experiment was then performed with reverse gavage. The pleopods, stomachs, and guts were collected from the shrimp at 0, 24, 48, and 72 h post-infection (hpi). Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods, stomachs, and guts of shrimp after LvPT dsRNA interference than in those of the controls at 48 and 72 hpi. Our results imply that LvPT plays an important role during WSSV infection of the digestive tract.
关键词Litopenaeus Vannamei Digestive Tract Peritrophin-like Protein Dsrna Interference Binding Specificity White Spot Syndrome Virus (Wssv) Reverse Gavage
DOI10.1007/s00343-017-6109-2
收录类别SCI
语种英语
WOS记录号WOS:000415810900023
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文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/143280
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
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GB/T 7714
Xie Shijun,Li Fuhua,Zhang Xiaojun,et al. Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2017,35(6):1524-1530.
APA Xie Shijun,Li Fuhua,Zhang Xiaojun,Zhang Jiquan,&Xiang Jianhai.(2017).Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,35(6),1524-1530.
MLA Xie Shijun,et al."Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 35.6(2017):1524-1530.
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