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Cloning and characterization of nitrate reductase gene in Ulva prolifera (Ulvophyceae, Chlorophyta)
Guo, Yang1,2,3; Wang, Hao Zhe4; Wu, Chun Hui1,2,3; Fu, Hui Hui1,2; Jiang, Peng1,2
2017-10-01
发表期刊JOURNAL OF PHYCOLOGY
卷号53期号:5页码:1035-1043
文章类型Article
摘要Ulva spp. dominates green tides around the world, which are occurring at an accelerated rate. The competitive nitrogen assimilation efficiency in Ulva is suggested to result in ecological success against other seaweeds. However, molecular characterization of genes involved in nitrogen assimilation has not been conducted. Here, we describe the identification of the nitrate reductase (NR) gene from a green seaweed Ulva prolifera, an alga which is responsible for the world's largest green tide in the Yellow Sea. Using rapid amplification of cDNA ends and genome walking, the NR gene from U.prolifera (UpNR) was cloned, which consisted of six introns and seven exons encoding 863 amino acids. According to sequence alignment, the NR in U.prolifera was shown to possess all five essential domains and 21 key invariant residues in plant NRs. The GC content of third codon position of UpNR (82.75%) was as high as those of green microalgae, and the intron number supported a potential loss issue from green microalga to land plant. Real-time quantitative PCR results showed that UpNR transcript level was induced by nitrate and repressed by ammonium, which could not be removed by addition of extra nitrate, indicating that U.prolifera preferred ammonium to nitrate. Urea would not repress NR transcription by itself, while it weakened the induction effect of nitrate, implying it possibly inhibited nitrate uptake rather than nitrate reduction. These results suggest the use of UpNR as a gene-sensor to probe the N assimilation process in green tides caused by Ulva.
关键词Ammonium Expression Analysis Gene Cloning Green Tide Nitrate Nitrate Reductase Ulva Prolifera
DOI10.1111/jpy.12556
收录类别SCI
语种英语
WOS记录号WOS:000413167600011
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文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/143236
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Univ Texas Dallas, Dept Biol Sci, Dallas, TX 75080 USA
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Guo, Yang,Wang, Hao Zhe,Wu, Chun Hui,et al. Cloning and characterization of nitrate reductase gene in Ulva prolifera (Ulvophyceae, Chlorophyta)[J]. JOURNAL OF PHYCOLOGY,2017,53(5):1035-1043.
APA Guo, Yang,Wang, Hao Zhe,Wu, Chun Hui,Fu, Hui Hui,&Jiang, Peng.(2017).Cloning and characterization of nitrate reductase gene in Ulva prolifera (Ulvophyceae, Chlorophyta).JOURNAL OF PHYCOLOGY,53(5),1035-1043.
MLA Guo, Yang,et al."Cloning and characterization of nitrate reductase gene in Ulva prolifera (Ulvophyceae, Chlorophyta)".JOURNAL OF PHYCOLOGY 53.5(2017):1035-1043.
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