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Functional characterization of hemocytes from Chinese mitten crab Eriocheir sinensis by flow cytometry
Jia, Zhihao1,4; Wang, Lingling2; Jiang, Shuai1; Sun, Mingzhe1,4; Wang, Mengqiang1; Yi, Qilin3; Song, Linsheng2,3
2017-10-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume69Pages:15-25
SubtypeArticle
AbstractHemocytes comprise a diversity of cell types with functional and structural heterogeneity, and they play key roles in the host defense of invertebrates. In the present study, the hemocytes from Chinese mitten crab Eriocheir sinensis were directly separated into two groups by flow cytometry. The hemocytes in P1 group were full of round and abundant granules with deeply staining cytoplasm, while P2 hemocytes were more diverse with a wide range of sizes and less granularity. Both P1 and P2 hemocytes exhibited phagocytic ability, but the phagocytic rate of P1 hemocytes increased which was significantly higher than that of P2 hemocytes after LPS stimulations. The levels of ROS production and intracellular Calcium as well as lysosome content were higher in P1 hemocytes than that in P2 hemocytes under both normal and immune-activated situations. The genes involved in phagocytosis, antimicrobial and antioxidant activities were mainly expressed in P1 hemocytes, while the genes involved in proPO activation system were highly expressed in P2 hemocytes. These results collectively suggested that P1 hemocytes were the main immunocompetent hemocytes in Chinese mitten crab and P2 hemocytes mainly participated in proPO activation system. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordEriocheir Sinensis Flow Cytometry Hemocytes Cell Sorting Immune Stimulation
DOI10.1016/j.fsi.2017.08.001
Indexed BySCI
Language英语
WOS IDWOS:000412960200003
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143225
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jia, Zhihao,Wang, Lingling,Jiang, Shuai,et al. Functional characterization of hemocytes from Chinese mitten crab Eriocheir sinensis by flow cytometry[J]. FISH & SHELLFISH IMMUNOLOGY,2017,69:15-25.
APA Jia, Zhihao.,Wang, Lingling.,Jiang, Shuai.,Sun, Mingzhe.,Wang, Mengqiang.,...&Song, Linsheng.(2017).Functional characterization of hemocytes from Chinese mitten crab Eriocheir sinensis by flow cytometry.FISH & SHELLFISH IMMUNOLOGY,69,15-25.
MLA Jia, Zhihao,et al."Functional characterization of hemocytes from Chinese mitten crab Eriocheir sinensis by flow cytometry".FISH & SHELLFISH IMMUNOLOGY 69(2017):15-25.
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