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Effects of an allelochemical in Phaeodactylum tricornutum filtrate on Heterosigma akashiwo: Morphological, physiological and growth effects
Wang, Rui1,2; Xue, Qiaona1; Wang, Jiangtao1; Tan, Liju1; Zhang, Qingchun3; Zhao, Yue3; Anderson, Donald M.4
2017-11-01
Source PublicationCHEMOSPHERE
Volume186Pages:527-534
SubtypeArticle
AbstractThe effects of an allelochemical extracted from the culture filtrate of diatom Phaeodactylum tricornutum on the raphidophyte Heterosigma akashiwo were investigated using a series of morphological, physiological and biochemical characters. Growth experiments showed that H. akashiwo was significantly inhibited immediately after exposure to the allelochemical, with many cells rapidly dying and lysing based on microscopic observation. The effects of the allelochemical on the surviving cells were explored using Scanning Electron Microscopy (SEM) and Flow cytometry (FCM), the latter by examination of a suite of physiological parameters (membrane integrity, esterase activity, chlorophyll-a content, membrane potential). The results demonstrate that the membrane of H. akashiwo was attacked by the allelochemical directly, causing cell membrane breakage and loss of integrity. Esterase activity was the most sensitive indicator of the impacts of the allelochemical. Membrane potential and chlorophyll-a content both showed significant decreases following exposure of the Heterosigma cells to high concentrations of the allelochemical for 5 and 6 days. Both were affected, but the membrane potential response was more gradual compared to other effects. The cell size of H. akashiwo did not change compared with the control group. The surviving cells were able to continue to grow and in a few days, re-establish a successful culture, even in the presence of residual allelochemical, suggesting either development of cellular resistance, or the degradation of the chemical. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordAllelochemical Phaeodactylum Tricornutum Heterosigma Akashiwo Flow Cytometry Physiological Characters
DOI10.1016/j.chemosphere.2017.08.024
Indexed BySCI
Language英语
WOS IDWOS:000411846900062
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143224
Collection海洋生态与环境科学重点实验室
Affiliation1.Ocean Univ China, Key Lab Marine Chem Theory & Technol, Minist Educ, Qingdao 266100, Peoples R China
2.City Univ Hong Kong, Res Ctr Oceans & Human Hlth, Shenzhen Key Lab Sustainable Use Marine Biodivers, Shenzhen Res Inst, Shenzhen 518057, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
4.Woods Hole Oceanog Inst, Biol Dept, Woods Hole, MA 02543 USA
Recommended Citation
GB/T 7714
Wang, Rui,Xue, Qiaona,Wang, Jiangtao,et al. Effects of an allelochemical in Phaeodactylum tricornutum filtrate on Heterosigma akashiwo: Morphological, physiological and growth effects[J]. CHEMOSPHERE,2017,186:527-534.
APA Wang, Rui.,Xue, Qiaona.,Wang, Jiangtao.,Tan, Liju.,Zhang, Qingchun.,...&Anderson, Donald M..(2017).Effects of an allelochemical in Phaeodactylum tricornutum filtrate on Heterosigma akashiwo: Morphological, physiological and growth effects.CHEMOSPHERE,186,527-534.
MLA Wang, Rui,et al."Effects of an allelochemical in Phaeodactylum tricornutum filtrate on Heterosigma akashiwo: Morphological, physiological and growth effects".CHEMOSPHERE 186(2017):527-534.
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