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Respiration disruption and detoxification at the protein expression levels in the Pacific oyster (&ITCrassostrea gigas&IT) under zinc exposure
Meng, Jie1,2,3; Wang, Wen-Xiong4; Li, Li1,2,3; Zhang, Guofan1,3,5
2017-10-01
Source PublicationAQUATIC TOXICOLOGY
Volume191Pages:34-41
SubtypeArticle
AbstractThe Pacific oyster (Crassostrea gigas) can accumulate high levels of zinc (Zn) without obvious toxicity, but the related molecular mechanisms are largely unknown. In the present study, C. gigas were exposed to excess Zn for 9 days and the differentially expressed proteins (DEPs) were examined using the isobarictags for relative and absolute quantitation (iTRAQ) method. In total, 2667 proteins containing at least two peptides and detected in both replicates were used for proteomic analysis. Among these DEPs, 332 were up-regulated and 282 were down-regulated. KEGG enrichment analysis of DEPs revealed that Zn exposure mainly distrubed 'tricarboxylic acid (TCA) cycle', 'electron transport chain (ETC)' and 'glutathione (GSH) metabolism' processes in oysters. Further key protein expressions enriched in these metabolism pathways were analyzed. In TCA cycle, Zn inhibited the Fe-containlng protein expressions, which may lead to the accumulation of succinate and induce anaerobiosis. In ETC metabolism process, Zn inhibited ETC complex protein expressions, including complex I-IV, which may affect the electron transport process. Furthermore, Zn induced phytochelatin (PC) and glutathione peroxidase (GPX) expression in GSH catabolism. The proteins play important roles in Zn detoxification and ROS elimination process. The transcriptional expressions of genes encoding these proteins were observed using real-time PCR analysis, and there was good consistency between these two datasets. Overall, we provide direct evidence for Zn toxicity and detoxification mechanisms at protein level.
DOI10.1016/j.aquatox.2017.07.011
Indexed BySCI
Language英语
WOS IDWOS:000411848700005
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143211
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao, Shandong, Peoples R China
3.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China
4.HKUST Shenzhen Res Inst, Marine Environm Lab, Shenzhen 518057, Peoples R China
5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
First Author Affilication中国科学院海洋研究所
Recommended Citation
GB/T 7714
Meng, Jie,Wang, Wen-Xiong,Li, Li,et al. Respiration disruption and detoxification at the protein expression levels in the Pacific oyster (&ITCrassostrea gigas&IT) under zinc exposure[J]. AQUATIC TOXICOLOGY,2017,191:34-41.
APA Meng, Jie,Wang, Wen-Xiong,Li, Li,&Zhang, Guofan.(2017).Respiration disruption and detoxification at the protein expression levels in the Pacific oyster (&ITCrassostrea gigas&IT) under zinc exposure.AQUATIC TOXICOLOGY,191,34-41.
MLA Meng, Jie,et al."Respiration disruption and detoxification at the protein expression levels in the Pacific oyster (&ITCrassostrea gigas&IT) under zinc exposure".AQUATIC TOXICOLOGY 191(2017):34-41.
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