中国科学院海洋研究所机构知识库
Advanced  
IOCAS-IR  > 海洋腐蚀与防护研究发展中心  > 期刊论文
题名: Identification of an MITF gene and its polymorphisms associated with the Vibrio resistance trait in the clam Meretrix petechialis
作者: Zhang, Shujing1,2; Yue, Xin1; Jiang, Fengjuan1,2; Wang, Hongxia1; Liu, Baozhong1,3
刊名: FISH & SHELLFISH IMMUNOLOGY
出版日期: 2017-09-01
卷号: 68, 页码:466-473
关键词: Meretrix petechialis ; MITF ; SNP ; Vibrio resistance
DOI: 10.1016/j.fsi.2017.07.035
文章类型: Article
英文摘要: Microphthalmia-associated transcription factor (MITF) regulates the transcription of its target genes by binding to their promoters. In this study, an MITF gene, MpMITF was identified in the clam Meretrix petechialis. The full-length cDNA of MpMITF is 3564 bp with an ORF of 1365 bp. The deduced amino acid sequence consists of a conserved functional structure of bHLH-LZ, which could bind with E-box. The mRNA and protein expression levels of MpMITF were significantly up-regulated 6 h post-Vibrio injection. The mRNA expression of MpMlTF increased on day 2 and peaked on day 10 post-Vibrio immersion. Furthermore, MpMITF expression was significantly up-regulated in most resistant families of clams (P < 0.05) but did not change significantly in most susceptive families of clams after the Vibrio immersion challenge. These results suggest that, in clams, MpMITF participates in the immune response against a Vibrio infection. Genotyping in two clam groups with different resistant levels to Vibrio parahaemolyticus (i.e., 11-R and 11-S), thirteen SNPs and five haplotypes were detected in the DNA sequence of MpMITF, of which five SNPs and two haplotypes were associated with Vibrio resistance. Four SNPs (SNP2, 5, 6 and 13) and one haplotype (Hap1) were further confirmed to be associated with Vibrio resistance in M. petechialis by association analysis in different clam families. This study deepens the understanding of MITF in marine bivalves and provides potential candidate markers for resistance selection in the clam M. petechialis. (C) 2017 Elsevier Ltd, All rights reserved.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000411299500051
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.qdio.ac.cn/handle/337002/143207
Appears in Collections:海洋腐蚀与防护研究发展中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Identification of an MITF gene and its polymorphisms associated with the Vibrio resistance trait in the clam Meretrix petechialis.pdf(931KB)期刊论文出版稿开放获取View 联系获取全文

作者单位: 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China

Recommended Citation:
Zhang, Shujing,Yue, Xin,Jiang, Fengjuan,et al. Identification of an MITF gene and its polymorphisms associated with the Vibrio resistance trait in the clam Meretrix petechialis[J]. FISH & SHELLFISH IMMUNOLOGY,2017,68:466-473.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, Shujing]'s Articles
[Yue, Xin]'s Articles
[Jiang, Fengjuan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, Shujing]‘s Articles
[Yue, Xin]‘s Articles
[Jiang, Fengjuan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Identification of an MITF gene and its polymorphisms associated with the Vibrio resistance trait in the clam Meretrix petechialis.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  中国科学院海洋研究所 - Feedback
Powered by CSpace