IOCAS-IR  > 海洋环境腐蚀与与生物污损重点实验室
Identification of an MITF gene and its polymorphisms associated with the Vibrio resistance trait in the clam Meretrix petechialis
Zhang, Shujing1,2; Yue, Xin1; Jiang, Fengjuan1,2; Wang, Hongxia1; Liu, Baozhong1,3
2017-09-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume68Pages:466-473
SubtypeArticle
AbstractMicrophthalmia-associated transcription factor (MITF) regulates the transcription of its target genes by binding to their promoters. In this study, an MITF gene, MpMITF was identified in the clam Meretrix petechialis. The full-length cDNA of MpMITF is 3564 bp with an ORF of 1365 bp. The deduced amino acid sequence consists of a conserved functional structure of bHLH-LZ, which could bind with E-box. The mRNA and protein expression levels of MpMITF were significantly up-regulated 6 h post-Vibrio injection. The mRNA expression of MpMlTF increased on day 2 and peaked on day 10 post-Vibrio immersion. Furthermore, MpMITF expression was significantly up-regulated in most resistant families of clams (P < 0.05) but did not change significantly in most susceptive families of clams after the Vibrio immersion challenge. These results suggest that, in clams, MpMITF participates in the immune response against a Vibrio infection. Genotyping in two clam groups with different resistant levels to Vibrio parahaemolyticus (i.e., 11-R and 11-S), thirteen SNPs and five haplotypes were detected in the DNA sequence of MpMITF, of which five SNPs and two haplotypes were associated with Vibrio resistance. Four SNPs (SNP2, 5, 6 and 13) and one haplotype (Hap1) were further confirmed to be associated with Vibrio resistance in M. petechialis by association analysis in different clam families. This study deepens the understanding of MITF in marine bivalves and provides potential candidate markers for resistance selection in the clam M. petechialis. (C) 2017 Elsevier Ltd, All rights reserved.
KeywordMeretrix Petechialis Mitf Snp Vibrio Resistance
DOI10.1016/j.fsi.2017.07.035
Indexed BySCI
Language英语
WOS IDWOS:000411299500051
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143207
Collection海洋环境腐蚀与与生物污损重点实验室
实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Shujing,Yue, Xin,Jiang, Fengjuan,et al. Identification of an MITF gene and its polymorphisms associated with the Vibrio resistance trait in the clam Meretrix petechialis[J]. FISH & SHELLFISH IMMUNOLOGY,2017,68:466-473.
APA Zhang, Shujing,Yue, Xin,Jiang, Fengjuan,Wang, Hongxia,&Liu, Baozhong.(2017).Identification of an MITF gene and its polymorphisms associated with the Vibrio resistance trait in the clam Meretrix petechialis.FISH & SHELLFISH IMMUNOLOGY,68,466-473.
MLA Zhang, Shujing,et al."Identification of an MITF gene and its polymorphisms associated with the Vibrio resistance trait in the clam Meretrix petechialis".FISH & SHELLFISH IMMUNOLOGY 68(2017):466-473.
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