| H-pylori alpha 1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides | |
| Yu, Hai1; Li, Yanhong1; Wu, Zhigang2,3; Li, Lei2,3; Zeng, Jie1,4; Zhao, Chao1,5; Wu, Yijing1,5; Tasnima, Nova1; Wang, Jing1,6; Liu, Huaide1,7; Gadi, Madhusudhan Reddy2,3; Guan, Wanyi2,3,8; Wang, Peng G.2,3; Chen, Xi1 | |
| 2017-10-14 | |
| 发表期刊 | CHEMICAL COMMUNICATIONS
 |
| 卷号 | 53期号:80页码:11012-11015 |
| 文章类型 | Article |
| 摘要 | Helicobacter pylori alpha 1-3/4-fucosyltransferase (Hp3/4FT) was expressed in Escherichia coli at a level of 30 mg L-1 culture and used as a diverse catalyst in a one-pot multienzyme (OPME) system for high-yield production of L-fucose-containing carbohydrates including Lewis antigens such as Lewis a, b, and x, O-sulfated Lewis x, and sialyl Lewis x and human milk fucosides such as 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP) III, and lacto-N-difuco-hexaose (LNDFH) II and III. Noticeably, while difucosylation of tetrasaccharides was readily achieved using an excess amount of donor, the synthesis of LNFP III was achieved by Hp3/4FT-catalyzed selective fucosylation of the N-acetyllactosamine (LacNAc) component in lacto-N-neotetraose (LNnT). |
| DOI | 10.1039/c7cc05403c |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000412410700007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/143194 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA 2.Georgia State Univ, Dept Chem, Atlanta, GA 30303 USA 3.Georgia State Univ, Ctr Diagnost & Therapeut, Atlanta, GA 30303 USA 4.Henan Inst Sci & Technol, Sch Food Sci, Xinxiang 453003, Henan, Peoples R China 5.Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 35002, Fujian, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 7.Nantong Univ, Sch Life Sci, Seyuan Rd 9, Nantong 226019, Peoples R China 8.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050024, Hebei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Hai,Li, Yanhong,Wu, Zhigang,et al. H-pylori alpha 1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides[J]. CHEMICAL COMMUNICATIONS,2017,53(80):11012-11015. |
| APA | Yu, Hai.,Li, Yanhong.,Wu, Zhigang.,Li, Lei.,Zeng, Jie.,...&Chen, Xi.(2017).H-pylori alpha 1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides.CHEMICAL COMMUNICATIONS,53(80),11012-11015. |
| MLA | Yu, Hai,et al."H-pylori alpha 1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides".CHEMICAL COMMUNICATIONS 53.80(2017):11012-11015. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| H-pylori alpha 1-3_4（631KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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