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H-pylori alpha 1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides
Yu, Hai1; Li, Yanhong1; Wu, Zhigang2,3; Li, Lei2,3; Zeng, Jie1,4; Zhao, Chao1,5; Wu, Yijing1,5; Tasnima, Nova1; Wang, Jing1,6; Liu, Huaide1,7; Gadi, Madhusudhan Reddy2,3; Guan, Wanyi2,3,8; Wang, Peng G.2,3; Chen, Xi1
2017-10-14
Source PublicationCHEMICAL COMMUNICATIONS
Volume53Issue:80Pages:11012-11015
SubtypeArticle
AbstractHelicobacter pylori alpha 1-3/4-fucosyltransferase (Hp3/4FT) was expressed in Escherichia coli at a level of 30 mg L-1 culture and used as a diverse catalyst in a one-pot multienzyme (OPME) system for high-yield production of L-fucose-containing carbohydrates including Lewis antigens such as Lewis a, b, and x, O-sulfated Lewis x, and sialyl Lewis x and human milk fucosides such as 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP) III, and lacto-N-difuco-hexaose (LNDFH) II and III. Noticeably, while difucosylation of tetrasaccharides was readily achieved using an excess amount of donor, the synthesis of LNFP III was achieved by Hp3/4FT-catalyzed selective fucosylation of the N-acetyllactosamine (LacNAc) component in lacto-N-neotetraose (LNnT).
DOI10.1039/c7cc05403c
Indexed BySCI
Language英语
WOS IDWOS:000412410700007
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143194
Collection海洋生物技术研发中心
Affiliation1.Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
2.Georgia State Univ, Dept Chem, Atlanta, GA 30303 USA
3.Georgia State Univ, Ctr Diagnost & Therapeut, Atlanta, GA 30303 USA
4.Henan Inst Sci & Technol, Sch Food Sci, Xinxiang 453003, Henan, Peoples R China
5.Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 35002, Fujian, Peoples R China
6.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
7.Nantong Univ, Sch Life Sci, Seyuan Rd 9, Nantong 226019, Peoples R China
8.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050024, Hebei, Peoples R China
Recommended Citation
GB/T 7714
Yu, Hai,Li, Yanhong,Wu, Zhigang,et al. H-pylori alpha 1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides[J]. CHEMICAL COMMUNICATIONS,2017,53(80):11012-11015.
APA Yu, Hai.,Li, Yanhong.,Wu, Zhigang.,Li, Lei.,Zeng, Jie.,...&Chen, Xi.(2017).H-pylori alpha 1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides.CHEMICAL COMMUNICATIONS,53(80),11012-11015.
MLA Yu, Hai,et al."H-pylori alpha 1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides".CHEMICAL COMMUNICATIONS 53.80(2017):11012-11015.
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