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Cadmium effects on DNA and protein metabolism in oyster (Crassostrea gigas) revealed by proteomic analyses
Meng, Jie1,3,4; Wang, Wenxiong5; Li, Li1,3,4; Yin, Qi6; Zhang, Guofan1,2,4
2017-09-15
Source PublicationSCIENTIFIC REPORTS
Volume7
SubtypeArticle
AbstractMarine molluscs, including oysters, can concentrate high levels of cadmium (Cd) in their soft tissues, but the molecular mechanisms of Cd toxicity remain speculative. In this study, Pacific oysters (Crassostrea gigas) were exposed to Cd for 9 days and their gills were subjected to proteomic analysis, which were further confirmed with transcriptomic analysis. A total of 4,964 proteins was quantified and 515 differentially expressed proteins were identified in response to Cd exposure. Gene Ontology enrichment analysis revealed that excess Cd affected the DNA and protein metabolism. Specifically, Cd toxicity resulted in the inhibition of DNA glycosylase and gap-filling and ligation enzymes expressions in base excision repair pathway, which may have decreased DNA repair capacity. At the protein level, Cd induced the heat shock protein response, initiation of protein refolding as well as degradation by ubiquitin proteasome pathway, among other effects. Excess Cd also induced antioxidant responses, particularly glutathione metabolism, which play important roles in Cd chelation and anti-oxidation. This study provided the first molecular mechanisms of Cd toxicity on DNA and protein metabolism at protein levels, and identified molecular biomarkers for Cd toxicity in oysters.
DOI10.1038/s41598-017-11894-7
Indexed BySCI
Language英语
WOS IDWOS:000410859400048
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143180
Collection海洋生物技术研发中心
实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266071, Shandong, Peoples R China
4.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China
5.HKUST Shenzhen Res Inst, Marine Environm Lab, Shenzhen 518057, Peoples R China
6.BGI Shenzhen, Shenzhen 518083, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Meng, Jie,Wang, Wenxiong,Li, Li,et al. Cadmium effects on DNA and protein metabolism in oyster (Crassostrea gigas) revealed by proteomic analyses[J]. SCIENTIFIC REPORTS,2017,7.
APA Meng, Jie,Wang, Wenxiong,Li, Li,Yin, Qi,&Zhang, Guofan.(2017).Cadmium effects on DNA and protein metabolism in oyster (Crassostrea gigas) revealed by proteomic analyses.SCIENTIFIC REPORTS,7.
MLA Meng, Jie,et al."Cadmium effects on DNA and protein metabolism in oyster (Crassostrea gigas) revealed by proteomic analyses".SCIENTIFIC REPORTS 7(2017).
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