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Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates
Huang, Baoyu1,2,4; Zhang, Linlin1,2,4; Du, Yishuai1,2,4; Xu, Fei1,2,4; Li, Li1,3,4; Zhang, Guofan1,2,4
2017-08-15
Source PublicationSCIENTIFIC REPORTS
Volume7
SubtypeArticle
AbstractDespite the mitochondrial antiviral signalling protein (MAVS)-dependent RIG-I-like receptor (RLR) signalling pathway in the cytosol plays an indispensable role in the antiviral immunity of the host, surprising little is known in invertebrates. Here we characterized the major members of RLR pathway and investigated their signal transduction a Molluscs. We show that genes involved in RLR pathway were significantly induced during virus challenge, including CgRIG-I-1, CgMAVS, CgTRAF6 (TNF receptor-associated factor 6), and CgIRFs (interferon regulatory factors. Similar to human RIG-I, oyster RIG-I-1 could bind poly(I:C) directly in vitro and interact with oyster MAVS via its caspase activation and recruitment domains. We also show that transmembrane domain-dependent self-association of CgMAVS may be crucial for its signalling and that CgMAVS can recruit the downstream signalling molecule, TRAF6, which can subsequently activate NF-kappa B signal pathway. Moreover, oyster IRFs appeared to function downstream of CgMAVS and were able to activate the interferon beta promoter and interferon stimulated response elements in mammalian cells. These results establish invertebrate MAVS-dependent RLR signalling for the first time and would be helpful for deciphering the antiviral mechanisms of invertebrates and understanding the development of the vertebrate RLR network.
DOI10.1038/s41598-017-08566-x
Indexed BySCI
Language英语
WOS IDWOS:000407570000094
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143149
Collection海洋生物技术研发中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
First Author Affilication中国科学院海洋研究所
Recommended Citation
GB/T 7714
Huang, Baoyu,Zhang, Linlin,Du, Yishuai,et al. Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates[J]. SCIENTIFIC REPORTS,2017,7.
APA Huang, Baoyu,Zhang, Linlin,Du, Yishuai,Xu, Fei,Li, Li,&Zhang, Guofan.(2017).Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates.SCIENTIFIC REPORTS,7.
MLA Huang, Baoyu,et al."Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates".SCIENTIFIC REPORTS 7(2017).
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