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High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention
Wang, Xueying1,2; Liu, Qinghua1,2; Xiao, Yongshuang1,2; Yang, Yang1,2,3; Wang, Yanfeng1,2; Song, Zongcheng4; You, Feng1,2; An, Hao1,2,3; Li, Jun1,2
2017-10-01
发表期刊AQUACULTURE
卷号479页码:808-816
文章类型Article
摘要Sex determination is not only resort to a single genetic cascade but also regulated along a continuum of environmental and heritable factors in many fish species. Temperature is one of the most important non-genetic sex differentiation elements. Previous studies regarding high temperature masculinization of flatfish typically focused on sex ratios and reproductive endocrinology, but little was known about germ cells during the process. In this study, we investigated the dynamic changes of germ cells in terms of morphology and number employing serial-sectioning and the molecular investigation of expression of dnd and amh genes by qRT-PCR for qualitative and quantitative analysis of germ cell proliferation during sexual differentiation. Moreover, the proliferation activity of primordial germ cells was investigated using PCNA (proliferating cell nuclear antigen) immunohistochemistry.
关键词Paralichthys Olivaceus High Temperature Germ Cell Sex Differentiation
DOI10.1016/j.aquaculture.2017.07.019
收录类别SCI
语种英语
WOS记录号WOS:000408034700099
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/143135
专题实验海洋生物学重点实验室
海洋生物技术研发中心
胶州湾海洋生态系统国家野外研究站
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China
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GB/T 7714
Wang, Xueying,Liu, Qinghua,Xiao, Yongshuang,et al. High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention[J]. AQUACULTURE,2017,479:808-816.
APA Wang, Xueying.,Liu, Qinghua.,Xiao, Yongshuang.,Yang, Yang.,Wang, Yanfeng.,...&Li, Jun.(2017).High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention.AQUACULTURE,479,808-816.
MLA Wang, Xueying,et al."High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention".AQUACULTURE 479(2017):808-816.
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