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Intracellular Trafficking Pathways of Edwardsiella tarda: From Clathrin- and Caveolin-Mediated Endocytosis to Endosome and Lysosome
Sui, Zhi-hai1,2,3; Xu, Haijiao4; Wang, Hongda4; Jiang, Shuai1,2; Chi, Heng1,2; Sun, Li1,2
2017-09-06
发表期刊FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷号7
文章类型Article
摘要Edwardsiella tarda is a Gram-negative bacterium that can infect a broad range of hosts including humans and fish. Accumulating evidences have indicated that E. tarda is able to survive and replicate in host phagocytes. However, the pathways involved in the intracellular infection of E. tarda are unclear. In this study, we examined the entry and endocytic trafficking of E. tarda in the mouse macrophage cell line RAW264.7. We found that E. tarda entered RAW264.7 and multiplied intracellularly in a robust manner. Cellular invasion of E. tarda was significantly impaired by inhibition of clathrin- and caveolin-mediated endocytic pathways and by inhibition of endosome acidification, but not by inhibition of macropinocytosis. Consistently, RAW264.7-infecting E. tarda was co-localized with clathrin, caveolin, and hallmarks of early and late endosomes, and intracellular E. tarda was found to exist in acid organelles. In addition, E. tarda in RAW264.7 was associated with actin and microtubule, and blocking of the functions of these cytoskeletons by inhibitors significantly decreased E. tarda infection. Furthermore, formaldehyde-killed E. tarda exhibited routes of cellular uptake and intracellular trafficking similar to that of live E. tarda. Together these results provide the first evidence that entry of live E. tarda into macrophages is probably a passive, virulence-independent process of phagocytosis effected by clathrin- and caveolin-mediated endocytosis and cytoskeletons, and that the intracellular traffic of E. tarda involves endosomes and endolysosomes.
关键词Edwardsiella Tarda Endocytosis Endosome Lysosome Cytoskeleton
DOI10.3389/fcimb.2017.00400
收录类别SCI
语种英语
WOS记录号WOS:000410041100002
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
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条目标识符http://ir.qdio.ac.cn/handle/337002/143120
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun, Jilin, Peoples R China
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Sui, Zhi-hai,Xu, Haijiao,Wang, Hongda,et al. Intracellular Trafficking Pathways of Edwardsiella tarda: From Clathrin- and Caveolin-Mediated Endocytosis to Endosome and Lysosome[J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2017,7.
APA Sui, Zhi-hai,Xu, Haijiao,Wang, Hongda,Jiang, Shuai,Chi, Heng,&Sun, Li.(2017).Intracellular Trafficking Pathways of Edwardsiella tarda: From Clathrin- and Caveolin-Mediated Endocytosis to Endosome and Lysosome.FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,7.
MLA Sui, Zhi-hai,et al."Intracellular Trafficking Pathways of Edwardsiella tarda: From Clathrin- and Caveolin-Mediated Endocytosis to Endosome and Lysosome".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 7(2017).
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