IOCAS-IR  > 实验海洋生物学重点实验室
Intracellular Trafficking Pathways of Edwardsiella tarda: From Clathrin- and Caveolin-Mediated Endocytosis to Endosome and Lysosome
Sui, Zhi-hai1,2,3; Xu, Haijiao4; Wang, Hongda4; Jiang, Shuai1,2; Chi, Heng1,2; Sun, Li1,2
2017-09-06
Source PublicationFRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
Volume7
SubtypeArticle
AbstractEdwardsiella tarda is a Gram-negative bacterium that can infect a broad range of hosts including humans and fish. Accumulating evidences have indicated that E. tarda is able to survive and replicate in host phagocytes. However, the pathways involved in the intracellular infection of E. tarda are unclear. In this study, we examined the entry and endocytic trafficking of E. tarda in the mouse macrophage cell line RAW264.7. We found that E. tarda entered RAW264.7 and multiplied intracellularly in a robust manner. Cellular invasion of E. tarda was significantly impaired by inhibition of clathrin- and caveolin-mediated endocytic pathways and by inhibition of endosome acidification, but not by inhibition of macropinocytosis. Consistently, RAW264.7-infecting E. tarda was co-localized with clathrin, caveolin, and hallmarks of early and late endosomes, and intracellular E. tarda was found to exist in acid organelles. In addition, E. tarda in RAW264.7 was associated with actin and microtubule, and blocking of the functions of these cytoskeletons by inhibitors significantly decreased E. tarda infection. Furthermore, formaldehyde-killed E. tarda exhibited routes of cellular uptake and intracellular trafficking similar to that of live E. tarda. Together these results provide the first evidence that entry of live E. tarda into macrophages is probably a passive, virulence-independent process of phagocytosis effected by clathrin- and caveolin-mediated endocytosis and cytoskeletons, and that the intracellular traffic of E. tarda involves endosomes and endolysosomes.
KeywordEdwardsiella Tarda Endocytosis Endosome Lysosome Cytoskeleton
DOI10.3389/fcimb.2017.00400
Indexed BySCI
Language英语
WOS IDWOS:000410041100002
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143120
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun, Jilin, Peoples R China
First Author Affilication中国科学院海洋研究所
Recommended Citation
GB/T 7714
Sui, Zhi-hai,Xu, Haijiao,Wang, Hongda,et al. Intracellular Trafficking Pathways of Edwardsiella tarda: From Clathrin- and Caveolin-Mediated Endocytosis to Endosome and Lysosome[J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2017,7.
APA Sui, Zhi-hai,Xu, Haijiao,Wang, Hongda,Jiang, Shuai,Chi, Heng,&Sun, Li.(2017).Intracellular Trafficking Pathways of Edwardsiella tarda: From Clathrin- and Caveolin-Mediated Endocytosis to Endosome and Lysosome.FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,7.
MLA Sui, Zhi-hai,et al."Intracellular Trafficking Pathways of Edwardsiella tarda: From Clathrin- and Caveolin-Mediated Endocytosis to Endosome and Lysosome".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 7(2017).
Files in This Item:
File Name/Size DocType Version Access License
Intracellular Traffi(2022KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Sui, Zhi-hai]'s Articles
[Xu, Haijiao]'s Articles
[Wang, Hongda]'s Articles
Baidu academic
Similar articles in Baidu academic
[Sui, Zhi-hai]'s Articles
[Xu, Haijiao]'s Articles
[Wang, Hongda]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Sui, Zhi-hai]'s Articles
[Xu, Haijiao]'s Articles
[Wang, Hongda]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Intracellular Trafficking Pathways of Edwardsiella tarda_ From Clathrin- and Caveolin-Mediated Endocytosis to Endosome and Lysosome.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.