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The fragmentation mechanism and immune-protective effect of CfTEP in the scallop Chlamys farreri
Xue, Zhuang1,2; Wang, Leilei3,4; Liu, Zhaoqun3; Wang, Weilin3; Liu, Chang1; Song, Xiaorui1; Wang, Lingling1; Song, Linsheng1,2
2017-11-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume76Pages:220-228
SubtypeArticle
AbstractThioester-containing proteins (TEPs), characterized by a unique intrachain beta-cysteinyl-gamma-glutamyl thioester bond, form an ancient and diverse family of secreted proteins that play central roles in the innate immune, response. But the existence form and immune protection mechanism of TEP in invertebrates still remain unclear, especially in the mollusks. The fragmentation and the immune-protective effect of thioester bond in CfTEP, a previously identified thioester-containing protein in scallop Chlamys farreri, were characterized in the present study. During the early embryonic development of scallop, the mRNA transcript of CfFEP could be detected in all the stages, and its expression levels in D-larvae, veliger larvae and eye-spot larvae were significantly higher than that in eggs. The CJTEP protein was also detected in peripheral of D-larvae, veliger larvae and eye-spot larva by immunofluorescence. In the adult scallop, the MEP protein was mainly distributed in the hepatopancreas, gill, kidney, gonad,.and mantle. The expression of CfrEP mRNA in the hemocytes of adult scallop was significantly up-regulated when the scallops were stimulated by LPS, PGN or beta-glucan. Two bands (100 and 55 kDa) were detected using antiCfTEP-R1 (spanned the C-terminal portion of the thioester, A2M-comp and A2M-recep domain, 942 1472), and a single band (46 kDa) was detected by using anti-CfFEP-R2 (the N-terminal portion of the following A2M-N-2 domain, 452-496) in the serum of scallop at 12 h after LPS stimulation. When the thioester bond of CfrEP protein was inactivated by injecting methylamine, the survival rate of scallop was significantly decreased after challenged by Vibrio angulillarum. All these results suggested that MEP protein existed as fragments similar to vertebrate C3, and played central roles in the immune response against pathogen in the innate immunity of scallops. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordChlamys Farreri Thioester-containing Proteins Cleavage Sites Innate Immunity Methylamine
DOI10.1016/j.dci.2017.06.005
Indexed BySCI
Language英语
WOS IDWOS:000407985100023
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143097
Collection实验海洋生物学重点实验室
Affiliation1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
4.Lianyungang Maternal & Child Hlth Hosp, Lianyungang 222000, Peoples R China
Recommended Citation
GB/T 7714
Xue, Zhuang,Wang, Leilei,Liu, Zhaoqun,et al. The fragmentation mechanism and immune-protective effect of CfTEP in the scallop Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,76:220-228.
APA Xue, Zhuang.,Wang, Leilei.,Liu, Zhaoqun.,Wang, Weilin.,Liu, Chang.,...&Song, Linsheng.(2017).The fragmentation mechanism and immune-protective effect of CfTEP in the scallop Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,76,220-228.
MLA Xue, Zhuang,et al."The fragmentation mechanism and immune-protective effect of CfTEP in the scallop Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 76(2017):220-228.
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