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Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida
Du, Yishuai1; Liu, Ying1; Xiao, Peng1; Meng, Lingjie1,2; Liu, Pengfei1,2
2017-08-01
Source PublicationJOURNAL OF THE WORLD AQUACULTURE SOCIETY
Volume48Issue:4Pages:574-582
SubtypeArticle
AbstractA rapid, economical, specific, and sensitive quantitative real-time polymerase chain reaction (qPCR) assay coupled with SYBR Green I chemistry was developed for the quantitative detection of Aeromonas salmonicida from farmed Atlantic salmon, Salmo salar, with the symptoms of furunculosis. The set of primers designed from the virulence array protein (vapA) gene was specific to A. salmonicida. Compared with the conventional PCR, qPCR had a lower detection limit of 5.6 copies of the positive plasmids. The standard curve, which showed the relationship between the copies of A. salmonicida and its quantification cycle (C-q) value, could be described as follows: log (copies of A. salmonicida)=-0.3213 C-q + 10.721. The quantitative detection of copies of A. salmonicida in different tissues of the moribund Atlantic salmon showed that A. salmonicida could be detected in all tissues; the spleen contained the largest number of A. salmonicida and then the kidney. These results suggest that the qPCR assay reported here is a specific, sensitive, and quantitative method for detecting A. salmonicida. It can be used for the routine tests of A. salmonicida in local aquaculture enterprise and for the research of infection routes of A. salmonicida to Atlantic salmon.
KeywordAtlantic Salmon Aeromonas Salmonicida Real-time Pcr Sybr Green i Quantitative Detection
DOI10.1111/jwas.12395
Indexed BySCI
Language英语
WOS IDWOS:000407075000005
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143089
Collection实验海洋生物学重点实验室
海洋生物技术研发中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Earth & Environm Sci Coll, Beijing 10049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Du, Yishuai,Liu, Ying,Xiao, Peng,et al. Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2017,48(4):574-582.
APA Du, Yishuai,Liu, Ying,Xiao, Peng,Meng, Lingjie,&Liu, Pengfei.(2017).Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,48(4),574-582.
MLA Du, Yishuai,et al."Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 48.4(2017):574-582.
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