Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Crystal structures of the isochorismatase domains from Vibrio anguillarum | |
| Du, Jiansen1,2; Deng, Tian1,2; Ma, Qingjun1,2 | |
| 2017-08-26 | |
| Source Publication | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| Volume | 490Issue:3Pages:827-833 |
| Subtype | Article |
| Abstract | Antibiotic resistance is becoming a global threat and overuse of antibiotics in aquaculture disease control worsens the situation. To reduce the risk of drug resistance developed in aquaculture, safer biocontrol programs are needed. Antivirulence therapy, with less chance for developing drug resistance, is a promising approach. To facilitate antivirulence inhibitor design against Vibrio anguillarum, a serious aquaculture pathogen, we present crystal structures for isochorismatase domains of AngB and VabB, which are required to synthesize siderophore, a critical virulence factor. Both structures are highly similar to known isochorismatases in fold and active site, therefore we conclude inhibitors for isochorismatases can be developed in a common framework. The structural information will improve design of virulence inhibitors against Vibrio anguillarum. We also firstly report that isochorismatase family could bind endogenous metabolite during the hetero-expression process, which is likely nicotinic acid, nicotinamide or pyrazinic acid, based on structural analysis and affinity prediction. Taken together, our results provide precise structural information of isochorismatase domains for antivirulence inhibitor design against Vibrio anguillarum. (C) 2017 Elsevier Inc. All rights reserved. |
| Keyword | Vibrio Isochorismatase Unknown Electron Density Antivirulence Inhibitor Aquaculture Disease |
| DOI | 10.1016/j.bbrc.2017.06.125 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000406820600037 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/143088 |
| Collection | 实验海洋生物学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Du, Jiansen,Deng, Tian,Ma, Qingjun. Crystal structures of the isochorismatase domains from Vibrio anguillarum[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2017,490(3):827-833. |
| APA | Du, Jiansen,Deng, Tian,&Ma, Qingjun.(2017).Crystal structures of the isochorismatase domains from Vibrio anguillarum.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,490(3),827-833. |
| MLA | Du, Jiansen,et al."Crystal structures of the isochorismatase domains from Vibrio anguillarum".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 490.3(2017):827-833. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Crystal structures o（2427KB） | 期刊论文 | 出版稿 | 延迟开放 | CC BY-NC-SA | View 2023-7-1后可获取 | |
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