Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus | |
| Wu, Shimei1; Zheng, Rikuan2,3,4; Sha, Zhenxia1; Sun, Chaomin2,4 | |
| 2017-07-01 | |
| 发表期刊 | MARINE DRUGS
 |
| 卷号 | 15期号:7 |
| 文章类型 | Article |
| 摘要 | Pseudomonas stutzeri 273 is a marine bacterium producing exopolysaccharide 273 (EPS273) with high anti-biofilm activity against P. aeruginosa PAO1. Here, the complete genome of P. stutzeri 273 was sequenced and the genome contained a circular 5.03 Mb chromosome. With extensive analysis of the genome, a genetic locus containing 18 genes was predicted to be involved in the biosynthesis of EPS273. In order to confirm this prediction, two adjacent genes (eps273-H and eps273-I) encoding glycosyltransferases and one gene (eps273-O) encoding tyrosine protein kinase within the genetic locus were deleted and biosynthesis of EPS273 was checked in parallel. The molecular weight profile of EPS purified from the mutant D eps273-HI was obviously different from that purified from wild-type P. stutzeri 273, while the corresponding EPS was hardly detected from the mutant D eps273-O, which indicated the involvement of the proposed 18-gene cluster in the biosynthesis of EPS273. Moreover, the mutant D eps273-HI had the biofilm formed earlier compared with the wild type, and the mutant D eps273-O almost completely lost the ability of biofilm formation. Therefore, EPS273 might facilitate the biofilm formation for its producing strain P. stutzeri 273 while inhibiting the biofilm formation of P. aeruginosa PAO1. This study can contribute to better understanding of the biosynthesis of EPS273 and disclose the biological function of EPS273 for its producing strain P. stutzeri 273. |
| 关键词 | Genome Pseudomonas Stutzeri Exopolysaccharide Biosynthesis Biofilm |
| DOI | 10.3390/md15070218 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000406685900026 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/143086 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Shimei,Zheng, Rikuan,Sha, Zhenxia,et al. Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus[J]. MARINE DRUGS,2017,15(7). |
| APA | Wu, Shimei,Zheng, Rikuan,Sha, Zhenxia,&Sun, Chaomin.(2017).Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus.MARINE DRUGS,15(7). |
| MLA | Wu, Shimei,et al."Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus".MARINE DRUGS 15.7(2017). |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Genome Sequence of P（4385KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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