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The RNA-seq analysis suggests a potential multi-component complement system in oyster Crassostrea gigas
Wang, Lingling1,2; Zhang, Huan3; Wang, Leilei3; Zhang, Daoxiang3; Lv, Zhao3; Liu, Zhaoqun3; Wang, Weilin3; Zhou, Zhi3; Qiu, Limei3; Wang, Hao3; Li, Jun4; Song, Linsheng1,2
2017-11-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume76Pages:209-219
SubtypeArticle
AbstractThe complement system is one of the major effector mechanisms of immune system, playing essential roles in both the innate and adaptive immune responses. In the present study, the counterparts of vertebrate complement components were identified by screening the sequenced genome of Crassostrea gigas, resulting in the identification of 792 gene models containing complement-related domains. The transcriptome of haemocytes at 6, 12 and 24 h post lipopolysaccharides (LPS) stimulation showed differential expression of 77 Clq domain containing proteins, 53 C-type lectins and 42 fibrinogen-related proteins. mRNAs encoding 18 serine protease domain-containing (SPC) proteins, 4 MACPF-domain containing proteins and 11 C3 receptor-like proteins were up-regulated upon LPS stimulation, and CgC3 mRNA was significantly increased at 12 h. The presence of CgC3 was confirmed in cell free plasma and was present in three subunit chains as expected for the processed mature protein. The complement related PRRs with coiled coil regions and SPC proteins with CUB domains may function in the activation of CgC3, whereas, the C3-like receptors with integrin-alpha/beta domain mediated the phagocytosis of C3-labled pathogens. These PRRs appear to serve as opsonins to promote phagocytosis of opsonized pathogens. The overall results suggested the existence of a potential multi-component complement system in C gigas. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordCrassostrea Gigas Complement Related Prrs Serine Protease Domain-containing Proteins C3 C3 Receptor-like Proteins
DOI10.1016/j.dci.2017.06.009
Indexed BySCI
Language英语
WOS IDWOS:000407985100022
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143083
Collection实验海洋生物学重点实验室
Affiliation1.Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
4.Lake Super State Univ, Sault Ste Marie, MI 49783 USA
Recommended Citation
GB/T 7714
Wang, Lingling,Zhang, Huan,Wang, Leilei,et al. The RNA-seq analysis suggests a potential multi-component complement system in oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,76:209-219.
APA Wang, Lingling.,Zhang, Huan.,Wang, Leilei.,Zhang, Daoxiang.,Lv, Zhao.,...&Song, Linsheng.(2017).The RNA-seq analysis suggests a potential multi-component complement system in oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,76,209-219.
MLA Wang, Lingling,et al."The RNA-seq analysis suggests a potential multi-component complement system in oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 76(2017):209-219.
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